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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Long-chain acyl-CoA thioesterase FadM (fadM) CSB-YP303548ENV
CSB-EP303548ENV
CSB-BP303548ENV
CSB-MP303548ENV
CSB-EP303548ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ydjF (ydjF) CSB-YP303549ENV
CSB-EP303549ENV
CSB-BP303549ENV
CSB-MP303549ENV
CSB-EP303549ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yniA (yniA) CSB-YP303550ENV
CSB-EP303550ENV
CSB-BP303550ENV
CSB-MP303550ENV
CSB-EP303550ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ydiJ (ydiJ), partial CSB-YP303551ENV
CSB-EP303551ENV
CSB-BP303551ENV
CSB-MP303551ENV
CSB-EP303551ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable dimethyl sulfoxide reductase chain ynfF (ynfF) CSB-YP303552ENV
CSB-EP303552ENV
CSB-BP303552ENV
CSB-MP303552ENV
CSB-EP303552ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yagX (yagX), partial CSB-YP303553ENV
CSB-EP303553ENV
CSB-BP303553ENV
CSB-MP303553ENV
CSB-EP303553ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides simplex Steroid Delta-isomerase (ksdI) CSB-YP303554NEB
CSB-EP303554NEB
CSB-BP303554NEB
CSB-MP303554NEB
CSB-EP303554NEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP303555GFM
CSB-EP303555GFM
CSB-BP303555GFM
CSB-MP303555GFM
CSB-EP303555GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Acetate kinase (ackA) CSB-YP303556DWY
CSB-EP303556DWY
CSB-BP303556DWY
CSB-MP303556DWY
CSB-EP303556DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Copper-transporting ATPase (copA), partial CSB-YP303557HUS
CSB-EP303557HUS
CSB-BP303557HUS
CSB-MP303557HUS
CSB-EP303557HUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Protein RecA (recA) CSB-YP303559EYA
CSB-EP303559EYA
CSB-BP303559EYA
CSB-MP303559EYA
CSB-EP303559EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces anulatus Peptidyl-prolyl cis-trans isomerase B (cypB) CSB-YP303560SNK
CSB-EP303560SNK
CSB-BP303560SNK
CSB-MP303560SNK
CSB-EP303560SNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Enolase (eno) CSB-YP303561SSQ
CSB-EP303561SSQ
CSB-BP303561SSQ
CSB-MP303561SSQ
CSB-EP303561SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae L-lactate dehydrogenase (ldh) CSB-YP303562MLW
CSB-EP303562MLW
CSB-BP303562MLW
CSB-MP303562MLW
CSB-EP303562MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Protein RecA (recA) CSB-YP303563MLW
CSB-EP303563MLW
CSB-BP303563MLW
CSB-MP303563MLW
CSB-EP303563MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae DNA ligase (ligA), partial CSB-YP303564MLW
CSB-EP303564MLW
CSB-BP303564MLW
CSB-MP303564MLW
CSB-EP303564MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Probable cation-transporting P-type ATPase (pacL), partial CSB-YP303565MLW
CSB-EP303565MLW
CSB-BP303565MLW
CSB-MP303565MLW
CSB-EP303565MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Catalase A (catA), partial CSB-YP303566NGS
CSB-EP303566NGS
CSB-BP303566NGS
CSB-MP303566NGS
CSB-EP303566NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Cytosine deaminase (FCA1) CSB-YP303567CZF
CSB-EP303567CZF
CSB-BP303567CZF
CSB-MP303567CZF
CSB-EP303567CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trametes hirsuta Ornithine carbamoyltransferase, mitochondrial, partial CSB-YP303568THK
CSB-EP303568THK
CSB-BP303568THK
CSB-MP303568THK
CSB-EP303568THK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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