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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP304759SUL
CSB-EP304759SUL
CSB-BP304759SUL
CSB-MP304759SUL
CSB-EP304759SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus deltoides Protein psbN (psbN), partial CSB-YP304760EXQ
CSB-EP304760EXQ
CSB-BP304760EXQ
CSB-MP304760EXQ
CSB-EP304760EXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein ipgE (ipgE) CSB-YP304761SGH
CSB-EP304761SGH
CSB-BP304761SGH
CSB-MP304761SGH
CSB-EP304761SGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA (Gln) amidotransferase subunit C CSB-YP304762SMT
CSB-EP304762SMT
CSB-BP304762SMT
CSB-MP304762SMT
CSB-EP304762SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S2 (rpsB) CSB-YP304763SMU
CSB-EP304763SMU
CSB-BP304763SMU
CSB-MP304763SMU
CSB-EP304763SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Mb2257 (Mb2257) CSB-YP304764MVH
CSB-EP304764MVH
CSB-BP304764MVH
CSB-MP304764MVH
CSB-EP304764MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0719 transmembrane protein Rv2600/MT2674.1 (Rv2600, MT2674.1), partial CSB-YP304765MVZ1
CSB-EP304765MVZ1
CSB-BP304765MVZ1
CSB-MP304765MVZ1
CSB-EP304765MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage VT2-Sa Antitermination protein Q (Q) CSB-YP304766EEC
CSB-EP304766EEC
CSB-BP304766EEC
CSB-MP304766EEC
CSB-EP304766EEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Lower collar protein (11) CSB-YP304767BBE
CSB-EP304767BBE
CSB-BP304767BBE
CSB-MP304767BBE
CSB-EP304767BBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant R-phycoerythrin beta chain (cpeB) CSB-YP304768EXX
CSB-EP304768EXX
CSB-BP304768EXX
CSB-MP304768EXX
CSB-EP304768EXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 1 Hexon-associated protein (PVIII) CSB-YP304769CCJ
CSB-EP304769CCJ
CSB-BP304769CCJ
CSB-MP304769CCJ
CSB-EP304769CCJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 1 Major core protein (PVII) CSB-YP304770CZQ
CSB-EP304770CZQ
CSB-BP304770CZQ
CSB-MP304770CZQ
CSB-EP304770CZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Hexon-interlacing protein (IX) CSB-YP304771HII
CSB-EP304771HII
CSB-BP304771HII
CSB-MP304771HII
CSB-EP304771HII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 11 Early E3 18.5 kDa glycoprotein, partial CSB-YP304772HIE
CSB-EP304772HIE
CSB-BP304772HIE
CSB-MP304772HIE
CSB-EP304772HIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens 50S ribosomal protein L30 (rpmD) CSB-YP304773AYS
CSB-EP304773AYS
CSB-BP304773AYS
CSB-MP304773AYS
CSB-EP304773AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Actin, cytoskeletal 2B (CYIIB) CSB-YP304774FOT
CSB-EP304774FOT
CSB-BP304774FOT
CSB-MP304774FOT
CSB-EP304774FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clupea pallasii Protamine-Z CSB-YP304775CMT
CSB-EP304775CMT
CSB-BP304775CMT
CSB-MP304775CMT
CSB-EP304775CMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria raniformis Aurein-2.5 CSB-YP304776LHP
CSB-EP304776LHP
CSB-BP304776LHP
CSB-MP304776LHP
CSB-EP304776LHP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria aurea Aurein-4.4 CSB-YP304777LHE
CSB-EP304777LHE
CSB-BP304777LHE
CSB-MP304777LHE
CSB-EP304777LHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protopolybia exigua Mastoparan-3 CSB-YP304778PSU
CSB-EP304778PSU
CSB-BP304778PSU
CSB-MP304778PSU
CSB-EP304778PSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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