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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Spinacia oleracea Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP304819FKI
CSB-EP304819FKI
CSB-BP304819FKI
CSB-MP304819FKI
CSB-EP304819FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP304820CTY
CSB-EP304820CTY
CSB-BP304820CTY
CSB-MP304820CTY
CSB-EP304820CTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber green mottle mosaic virus Capsid protein (CP) CSB-YP304821CTU
CSB-EP304821CTU
CSB-BP304821CTU
CSB-MP304821CTU
CSB-EP304821CTU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus C Intermediate capsid protein VP6 CSB-YP304822RHK
CSB-EP304822RHK
CSB-BP304822RHK
CSB-MP304822RHK
CSB-EP304822RHK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus imperialis Conotoxin Im11.1 CSB-YP304823DWA
CSB-EP304823DWA
CSB-BP304823DWA
CSB-MP304823DWA
CSB-EP304823DWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato mosaic virus Movement protein (MP) CSB-YP304824TGG
CSB-EP304824TGG
CSB-BP304824TGG
CSB-MP304824TGG
CSB-EP304824TGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable poly-beta-1,6-N-acetyl-D-glucosamine export protein (icaC), partial CSB-YP304825FLL
CSB-EP304825FLL
CSB-BP304825FLL
CSB-MP304825FLL
CSB-EP304825FLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage f1 Tail virion protein G7P (VII), partial CSB-YP304826ECH
CSB-EP304826ECH
CSB-BP304826ECH
CSB-MP304826ECH
CSB-EP304826ECH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage alpha3 Small core protein (J) CSB-YP304827ECD
CSB-EP304827ECD
CSB-BP304827ECD
CSB-MP304827ECD
CSB-EP304827ECD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Photosystem II reaction center protein H (psbH), partial CSB-YP304828TQN1
CSB-EP304828TQN1
CSB-BP304828TQN1
CSB-MP304828TQN1
CSB-EP304828TQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana plumbaginifolia Photosystem Q (B) protein, partial CSB-YP304829NHC1
CSB-EP304829NHC1
CSB-BP304829NHC1
CSB-MP304829NHC1
CSB-EP304829NHC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Euphorbia dulcis Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP304830EIF
CSB-EP304830EIF
CSB-BP304830EIF
CSB-MP304830EIF
CSB-EP304830EIF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus arcticus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP304831LJT
CSB-EP304831LJT
CSB-BP304831LJT
CSB-MP304831LJT
CSB-EP304831LJT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus micranthus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP304832LKB
CSB-EP304832LKB
CSB-BP304832LKB
CSB-MP304832LKB
CSB-EP304832LKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus polyphyllus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP304833LKJ
CSB-EP304833LKJ
CSB-BP304833LKJ
CSB-MP304833LKJ
CSB-EP304833LKJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Nucleoprotein (N) CSB-YP304834ARN
CSB-EP304834ARN
CSB-BP304834ARN
CSB-MP304834ARN
CSB-EP304834ARN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Membrane protein (M), partial CSB-YP304835ARO1
CSB-EP304835ARO1
CSB-BP304835ARO1
CSB-MP304835ARO1
CSB-EP304835ARO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine respiratory coronavirus Envelope small membrane protein (E), partial CSB-YP304836PQE1
CSB-EP304836PQE1
CSB-BP304836PQE1
CSB-MP304836PQE1
CSB-EP304836PQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3) CSB-YP304837HGE
CSB-EP304837HGE
CSB-BP304837HGE
CSB-MP304837HGE
CSB-EP304837HGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Uncharacterized 9.7 kDa protein in cox-rep intergenic region CSB-YP304838HAN
CSB-EP304838HAN
CSB-BP304838HAN
CSB-MP304838HAN
CSB-EP304838HAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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