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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Nuclease C1 (NUC1CE) CSB-YP305488CUO
CSB-EP305488CUO
CSB-BP305488CUO
CSB-MP305488CUO
CSB-EP305488CUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 21 (ACP21) CSB-YP305489LHY
CSB-EP305489LHY
CSB-BP305489LHY
CSB-MP305489LHY
CSB-EP305489LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Juniperus ashei Pathogenesis-related protein CSB-YP305490JAN
CSB-EP305490JAN
CSB-BP305490JAN
CSB-MP305490JAN
CSB-EP305490JAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0047.1 (MJ0047.1) CSB-YP305491MRU
CSB-EP305491MRU
CSB-BP305491MRU
CSB-MP305491MRU
CSB-EP305491MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0458.1 (MJ0458.1) CSB-YP305492MRU
CSB-EP305492MRU
CSB-BP305492MRU
CSB-MP305492MRU
CSB-EP305492MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ0792.1 (MJ0792.1), partial CSB-YP305493MRU1
CSB-EP305493MRU1
CSB-BP305493MRU1
CSB-MP305493MRU1
CSB-EP305493MRU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1288.1 (MJ1288.1), partial CSB-YP305494MRU
CSB-EP305494MRU
CSB-BP305494MRU
CSB-MP305494MRU
CSB-EP305494MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Uncharacterized protein MJ1597.1 (MJ1597.1) CSB-YP305495MRU
CSB-EP305495MRU
CSB-BP305495MRU
CSB-MP305495MRU
CSB-EP305495MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Acetyl-CoA decarbonylase/synthase complex subunit epsilon (cdhB) CSB-YP305496MRU
CSB-EP305496MRU
CSB-BP305496MRU
CSB-MP305496MRU
CSB-EP305496MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP305497CMA
CSB-EP305497CMA
CSB-BP305497CMA
CSB-MP305497CMA
CSB-EP305497CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Unknown protein CP 4 from 2D-PAGE CSB-YP305498CMA
CSB-EP305498CMA
CSB-BP305498CMA
CSB-MP305498CMA
CSB-EP305498CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-hydroxybutyryl-CoA dehydratase (crt) CSB-YP305499CMA
CSB-EP305499CMA
CSB-BP305499CMA
CSB-MP305499CMA
CSB-EP305499CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erythrina variegata Trypsin inhibitor DE-3 CSB-YP305500EGS
CSB-EP305500EGS
CSB-BP305500EGS
CSB-MP305500EGS
CSB-EP305500EGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorghum bicolor Alpha-amylase inhibitor 5 CSB-YP305501FJS
CSB-EP305501FJS
CSB-BP305501FJS
CSB-MP305501FJS
CSB-EP305501FJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein sspM (sspM) CSB-YP305502MVV
CSB-EP305502MVV
CSB-BP305502MVV
CSB-MP305502MVV
CSB-EP305502MVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antirrhinum majus Myb-related protein 330 (MYB330) CSB-YP305503DNE
CSB-EP305503DNE
CSB-BP305503DNE
CSB-MP305503DNE
CSB-EP305503DNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) CSB-YP305504EWE
CSB-EP305504EWE
CSB-BP305504EWE
CSB-MP305504EWE
CSB-EP305504EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Pyruvate dehydrogenase E1 component subunit beta, mitochondrial CSB-YP305505FIG
CSB-EP305505FIG
CSB-BP305505FIG
CSB-MP305505FIG
CSB-EP305505FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myrmecia gulosa Formaecin-1 CSB-YP305506MML
CSB-EP305506MML
CSB-BP305506MML
CSB-MP305506MML
CSB-EP305506MML-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Neutrophil defensin 2 CSB-YP305507MRG
CSB-EP305507MRG
CSB-BP305507MRG
CSB-MP305507MRG
CSB-EP305507MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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