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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Anticarsia gemmatalis nuclear polyhedrosis virus Late expression factor 2 (LEF-2) CSB-YP305508ALR
CSB-EP305508ALR
CSB-BP305508ALR
CSB-MP305508ALR
CSB-EP305508ALR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trimeresurus gramineus Phospholipase A2 isozyme 4 CSB-YP305509TQK
CSB-EP305509TQK
CSB-BP305509TQK
CSB-MP305509TQK
CSB-EP305509TQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phyllomedusa bicolor Dermaseptin-B5 CSB-YP305510PIP
CSB-EP305510PIP
CSB-BP305510PIP
CSB-MP305510PIP
CSB-EP305510PIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus horridus horridus CHH-B subunit beta CSB-YP305511CSY
CSB-EP305511CSY
CSB-BP305511CSY
CSB-MP305511CSY
CSB-EP305511CSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella piscicida Superoxide dismutase [Fe] (sodB) CSB-YP305512PDM
CSB-EP305512PDM
CSB-BP305512PDM
CSB-MP305512PDM
CSB-EP305512PDM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc-containing ferredoxin A (zfx) CSB-YP305513FPI
CSB-EP305513FPI
CSB-BP305513FPI
CSB-MP305513FPI
CSB-EP305513FPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Repressor-like protein SSo7c4 (SSO0046.1) CSB-YP305514FPM
CSB-EP305514FPM
CSB-BP305514FPM
CSB-MP305514FPM
CSB-EP305514FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Defensin D4 CSB-YP305515FKI
CSB-EP305515FKI
CSB-BP305515FKI
CSB-MP305515FKI
CSB-EP305515FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cancer pagurus Cuticle protein AM1274 CSB-YP305516CCE
CSB-EP305516CCE
CSB-BP305516CCE
CSB-MP305516CCE
CSB-EP305516CCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cancer pagurus Cuticle protein CP498 CSB-YP305517CCE
CSB-EP305517CCE
CSB-BP305517CCE
CSB-MP305517CCE
CSB-EP305517CCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfitobacterium hafniense 3-chloro-4-hydroxyphenylacetate reductive dehalogenase (cprA) CSB-YP305518DJJ
CSB-EP305518DJJ
CSB-BP305518DJJ
CSB-MP305518DJJ
CSB-EP305518DJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadronyche versuta Omega-hexatoxin-Hv1d CSB-YP305519HAD
CSB-EP305519HAD
CSB-BP305519HAD
CSB-MP305519HAD
CSB-EP305519HAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Salivary lipocalin (SAL1) CSB-YP305520PI
CSB-EP305520PI
CSB-BP305520PI
CSB-MP305520PI
CSB-EP305520PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus edulis Mytilin-B CSB-YP305521MWU
CSB-EP305521MWU
CSB-BP305521MWU
CSB-MP305521MWU
CSB-EP305521MWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Hypertrehalosaemic hormone CSB-YP305522LHY
CSB-EP305522LHY
CSB-BP305522LHY
CSB-MP305522LHY
CSB-EP305522LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echis carinatus Disintegrin EC3B CSB-YP305523EAH
CSB-EP305523EAH
CSB-BP305523EAH
CSB-MP305523EAH
CSB-EP305523EAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lentinula edodes Serine protease inhibitor CSB-YP305524LDV
CSB-BP305524LDV
CSB-MP305524LDV
CSB-EP305524LDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polistes dominula Venom allergen 5 CSB-YP305525POG
CSB-EP305525POG
CSB-BP305525POG
CSB-MP305525POG
CSB-EP305525POG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus pinaster Phosphoribulokinase CSB-YP305526PKM
CSB-EP305526PKM
CSB-BP305526PKM
CSB-MP305526PKM
CSB-EP305526PKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penaeus japonicus Crustacean hyperglycemic hormone 6 CSB-YP305527ETE
CSB-EP305527ETE
CSB-BP305527ETE
CSB-MP305527ETE
CSB-EP305527ETE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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