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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Allergen Asp fl 2 CSB-YP306532AVD
CSB-EP306532AVD
CSB-BP306532AVD
CSB-MP306532AVD
CSB-EP306532AVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana temporaria Brevinin-2Tb CSB-YP306533RBJ
CSB-EP306533RBJ
CSB-BP306533RBJ
CSB-MP306533RBJ
CSB-EP306533RBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombina variegata Bombinin-H1/H3 CSB-YP306534BIC
CSB-EP306534BIC
CSB-BP306534BIC
CSB-MP306534BIC
CSB-EP306534BIC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Hemocyanin subunit 4 CSB-YP306535HWO
CSB-EP306535HWO
CSB-BP306535HWO
CSB-MP306535HWO
CSB-EP306535HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maia squinado Hemocyanin subunit 4e CSB-YP306536MBF
CSB-EP306536MBF
CSB-BP306536MBF
CSB-MP306536MBF
CSB-EP306536MBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Neutrophil defensin 2 CSB-YP306537MOW
CSB-EP306537MOW
CSB-BP306537MOW
CSB-MP306537MOW
CSB-EP306537MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Unknown protein from spot 106 of 2D-PAGE of thylakoid CSB-YP306538EWE
CSB-EP306538EWE
CSB-BP306538EWE
CSB-MP306538EWE
CSB-EP306538EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Unknown protein from spot 112 of 2D-PAGE of thylakoid CSB-YP306539EWE
CSB-EP306539EWE
CSB-BP306539EWE
CSB-MP306539EWE
CSB-EP306539EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Unknown protein from spot 204 of 2D-PAGE of thylakoid CSB-YP306540EWE
CSB-EP306540EWE
CSB-BP306540EWE
CSB-MP306540EWE
CSB-EP306540EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria raniformis Aurein-1.1 CSB-YP306541LHP
CSB-EP306541LHP
CSB-BP306541LHP
CSB-MP306541LHP
CSB-EP306541LHP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria aurea Aurein-4.3 CSB-YP306542LHE
CSB-EP306542LHE
CSB-BP306542LHE
CSB-MP306542LHE
CSB-EP306542LHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pachycondyla goeldii Ponericin-G1 CSB-YP306543PAC
CSB-EP306543PAC
CSB-BP306543PAC
CSB-MP306543PAC
CSB-EP306543PAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pachycondyla goeldii Ponericin-L2 CSB-YP306544PAC
CSB-EP306544PAC
CSB-BP306544PAC
CSB-MP306544PAC
CSB-EP306544PAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pachycondyla goeldii Ponericin-W5 CSB-YP306545PAC
CSB-EP306545PAC
CSB-BP306545PAC
CSB-MP306545PAC
CSB-EP306545PAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 68 kDa cell wall protein CSB-YP306546NHE
CSB-EP306546NHE
CSB-BP306546NHE
CSB-MP306546NHE
CSB-EP306546NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echis carinatus sochureki Disintegrin EC6A CSB-YP306547EAI
CSB-EP306547EAI
CSB-BP306547EAI
CSB-MP306547EAI
CSB-EP306547EAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Thylakoid lumenal 17 kDa protein CSB-YP306548FKI
CSB-EP306548FKI
CSB-BP306548FKI
CSB-MP306548FKI
CSB-EP306548FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haliotis laevigata Perlustrin CSB-YP306549HAZ
CSB-EP306549HAZ
CSB-BP306549HAZ
CSB-MP306549HAZ
CSB-EP306549HAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Periplaneta americana Pyrokinin-5 CSB-YP306550PFW
CSB-EP306550PFW
CSB-BP306550PFW
CSB-MP306550PFW
CSB-EP306550PFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Defensin-like protein 246 (SCRL5) CSB-YP306551DOA
CSB-EP306551DOA
CSB-BP306551DOA
CSB-MP306551DOA
CSB-EP306551DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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