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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Litopenaeus vannamei Histone H2B CSB-YP307136LHD
CSB-EP307136LHD
CSB-BP307136LHD
CSB-MP307136LHD
CSB-EP307136LHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Subpeptin JM4-A CSB-YP307137BRI
CSB-EP307137BRI
CSB-BP307137BRI
CSB-MP307137BRI
CSB-EP307137BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria keyserlingi U1-ctenitoxin-Pk1a CSB-YP307138PHW
CSB-EP307138PHW
CSB-BP307138PHW
CSB-MP307138PHW
CSB-EP307138PHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria reidyi Omega-ctenitoxin-Pr1a CSB-YP307139PHX
CSB-EP307139PHX
CSB-BP307139PHX
CSB-MP307139PHX
CSB-EP307139PHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria keyserlingi U3-ctenitoxin-Pk1a CSB-YP307140PHW
CSB-EP307140PHW
CSB-BP307140PHW
CSB-MP307140PHW
CSB-EP307140PHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupiennius salei U2-ctenitoxin-Cs1a CSB-YP307141CUR
CSB-EP307141CUR
CSB-BP307141CUR
CSB-MP307141CUR
CSB-EP307141CUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gromphadorina portentosa Periviscerokinin-1 CSB-YP307142GDM
CSB-EP307142GDM
CSB-BP307142GDM
CSB-MP307142GDM
CSB-EP307142GDM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhyparobia maderae Periviscerokinin-3 CSB-YP307143REJ
CSB-EP307143REJ
CSB-BP307143REJ
CSB-MP307143REJ
CSB-EP307143REJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces exfoliatus Protein rarD (rarD), partial CSB-YP307144SOC
CSB-EP307144SOC
CSB-BP307144SOC
CSB-MP307144SOC
CSB-EP307144SOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptodactylus ocellatus Ocellatin-1 CSB-YP307145LEK
CSB-EP307145LEK
CSB-BP307145LEK
CSB-MP307145LEK
CSB-EP307145LEK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinidia chinensis Thaumatin-like protein (tlp) CSB-YP307146AXE
CSB-EP307146AXE
CSB-BP307146AXE
CSB-MP307146AXE
CSB-EP307146AXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acipenser transmontanus Ventricular natriuretic peptide (vnp) CSB-YP307147AWZ
CSB-EP307147AWZ
CSB-BP307147AWZ
CSB-MP307147AWZ
CSB-EP307147AWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Plasma membrane ATPase (ha1), partial CSB-YP307148TQN
CSB-EP307148TQN
CSB-BP307148TQN
CSB-MP307148TQN
CSB-EP307148TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ginglymostoma cirratum IgW transmembrane form Tm1T3/Tm6T3/Tm3C4, partial CSB-YP307149GCE
CSB-EP307149GCE
CSB-BP307149GCE
CSB-MP307149GCE
CSB-EP307149GCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 10.6 CSB-YP307150LHY
CSB-EP307150LHY
CSB-BP307150LHY
CSB-MP307150LHY
CSB-EP307150LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria nigriventer U19-ctenitoxin-Pn1a CSB-YP307151EUV
CSB-EP307151EUV
CSB-BP307151EUV
CSB-MP307151EUV
CSB-EP307151EUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ancylometes sp. U3-ctenitoxin-Asp1a CSB-YP307152AJW
CSB-EP307152AJW
CSB-BP307152AJW
CSB-MP307152AJW
CSB-EP307152AJW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria nigriventer U13-ctenitoxin-Pn1c CSB-YP307153EUV
CSB-EP307153EUV
CSB-BP307153EUV
CSB-MP307153EUV
CSB-EP307153EUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ancylometes sp. Delta-ctenitoxin-Asp2e CSB-YP307154AJW
CSB-EP307154AJW
CSB-BP307154AJW
CSB-MP307154AJW
CSB-EP307154AJW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria nigriventer U21-ctenitoxin-Pn1a CSB-YP307155EUV
CSB-EP307155EUV
CSB-BP307155EUV
CSB-MP307155EUV
CSB-EP307155EUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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