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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pneumoniae Uncharacterized protein SPD_2302 homolog (orf2) CSB-YP313878FMW
CSB-EP313878FMW
CSB-BP313878FMW
CSB-MP313878FMW
CSB-EP313878FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-DR6 Gag-Pol polyprotein (TY1B-DR6), partial CSB-YP313879SVG
CSB-EP313879SVG
CSB-BP313879SVG
CSB-MP313879SVG
CSB-EP313879SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty2-GR1 Gag polyprotein (TY2A-GR1) CSB-YP313880SVG
CSB-EP313880SVG
CSB-BP313880SVG
CSB-MP313880SVG
CSB-EP313880SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable thiol peroxidase (tpx) CSB-YP313881FMW
CSB-EP313881FMW
CSB-BP313881FMW
CSB-MP313881FMW
CSB-EP313881FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxosceles gaucho Sphingomyelin phosphodiesterase D LgSicTox-beta-LOXN2 CSB-YP313882LJE
CSB-EP313882LJE
CSB-BP313882LJE
CSB-MP313882LJE
CSB-EP313882LJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxosceles intermedia Sphingomyelin phosphodiesterase D LiSicTox-alphaI-1 CSB-YP313883LRA
CSB-EP313883LRA
CSB-BP313883LRA
CSB-MP313883LRA
CSB-EP313883LRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadronyche formidabilis Kappa-hexatoxin-Hf1a CSB-YP313884HAA
CSB-EP313884HAA
CSB-BP313884HAA
CSB-MP313884HAA
CSB-EP313884HAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M), partial CSB-YP313885IFJ1
CSB-EP313885IFJ1
CSB-BP313885IFJ1
CSB-MP313885IFJ1
CSB-EP313885IFJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23 (rplW) CSB-YP313886YAQ
CSB-EP313886YAQ
CSB-BP313886YAQ
CSB-MP313886YAQ
CSB-EP313886YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation protein X (yscX) CSB-YP313887YAQ
CSB-EP313887YAQ
CSB-BP313887YAQ
CSB-MP313887YAQ
CSB-EP313887YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Grammostola rosea Kappa-theraphotoxin-Gr2b CSB-YP313888GDE
CSB-EP313888GDE
CSB-BP313888GDE
CSB-MP313888GDE
CSB-EP313888GDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Envelope small membrane protein (E), partial CSB-YP313889BJL1
CSB-EP313889BJL1
CSB-BP313889BJL1
CSB-MP313889BJL1
CSB-EP313889BJL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Non-structural protein 2a (2a) CSB-YP313890BJI
CSB-MP313890BJI
CSB-EP313890BJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Non-structural protein of 4.8 kDa (4b) CSB-YP313891BJM
CSB-EP313891BJM
CSB-BP313891BJM
CSB-MP313891BJM
CSB-EP313891BJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endoglucanase A (celA) CSB-YP313892CMO
CSB-EP313892CMO
CSB-BP313892CMO
CSB-MP313892CMO
CSB-EP313892CMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antigen 85-B (fbpB) CSB-YP313893MVH
CSB-EP313893MVH
CSB-BP313893MVH
CSB-MP313893MVH
CSB-EP313893MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris L-lactate dehydrogenase 2 (ldhB) CSB-YP313894LNF
CSB-EP313894LNF
CSB-BP313894LNF
CSB-MP313894LNF
CSB-EP313894LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Ornithine carbamoyltransferase, catabolic (arcB) CSB-YP313895LNG
CSB-EP313895LNG
CSB-BP313895LNG
CSB-MP313895LNG
CSB-EP313895LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lycosa singoriensis M-lycotoxin-Ls3a CSB-YP313896LRO
CSB-EP313896LRO
CSB-BP313896LRO
CSB-MP313896LRO
CSB-EP313896LRO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Low calcium response locus protein D (lcrD), partial CSB-YP313897YAQ1
CSB-EP313897YAQ1
CSB-BP313897YAQ1
CSB-MP313897YAQ1
CSB-EP313897YAQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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