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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aspergillus terreus Blasticidin-S deaminase (bsd) CSB-YP314694APT
CSB-EP314694APT
CSB-BP314694APT
CSB-MP314694APT
CSB-EP314694APT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Grammostola rosea Kappa-theraphotoxin-Gr4a CSB-YP314695GDE
CSB-EP314695GDE
CSB-BP314695GDE
CSB-MP314695GDE
CSB-EP314695GDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Envelope small membrane protein (E), partial CSB-YP314696BJM1
CSB-EP314696BJM1
CSB-BP314696BJM1
CSB-MP314696BJM1
CSB-EP314696BJM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Non-structural protein of 4.8 kDa (4b) CSB-EP314697BJF
CSB-BP314697BJF
CSB-MP314697BJF
CSB-EP314697BJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endoglucanase SS (celS), partial CSB-YP314698CMO
CSB-EP314698CMO
CSB-BP314698CMO
CSB-MP314698CMO
CSB-EP314698CMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phoneutria nigriventer U12-ctenitoxin-Pn1a CSB-YP314699EUV
CSB-EP314699EUV
CSB-BP314699EUV
CSB-MP314699EUV
CSB-EP314699EUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Probable N-acetylmuramidase (acmA) CSB-YP314700LNF
CSB-EP314700LNF
CSB-BP314700LNF
CSB-MP314700LNF
CSB-EP314700LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Di-/tripeptide transporter (dtpT), partial CSB-YP314701LNF
CSB-EP314701LNF
CSB-BP314701LNF
CSB-MP314701LNF
CSB-EP314701LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lycosa singoriensis U1-lycotoxin-Ls1a CSB-YP314702LRO
CSB-EP314702LRO
CSB-BP314702LRO
CSB-MP314702LRO
CSB-EP314702LRO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Low calcium response locus protein R (lcrR) CSB-YP314703YAQ
CSB-EP314703YAQ
CSB-BP314703YAQ
CSB-MP314703YAQ
CSB-EP314703YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adhesin yadA (yadA) CSB-YP314704YAQ
CSB-EP314704YAQ
CSB-BP314704YAQ
CSB-MP314704YAQ
CSB-EP314704YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chilobrachys jingzhao U1-theraphotoxin-Cj1a CSB-YP314705DRU
CSB-EP314705DRU
CSB-BP314705DRU
CSB-MP314705DRU
CSB-EP314705DRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP314706OCO1
CSB-EP314706OCO1
CSB-BP314706OCO1
CSB-MP314706OCO1
CSB-EP314706OCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Uncharacterized protein ycf68 (ycf68-1) CSB-YP314707OFF
CSB-EP314707OFF
CSB-BP314707OFF
CSB-MP314707OFF
CSB-EP314707OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP314708OCO1
CSB-EP314708OCO1
CSB-BP314708OCO1
CSB-MP314708OCO1
CSB-EP314708OCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Riboflavin biosynthesis protein ribF (ribF) CSB-YP314709EGX
CSB-EP314709EGX
CSB-BP314709EGX
CSB-MP314709EGX
CSB-EP314709EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L30 (rpmD) CSB-YP314710EGX
CSB-EP314710EGX
CSB-BP314710EGX
CSB-MP314710EGX
CSB-EP314710EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S14 (rpsN) CSB-YP314711SZB
CSB-EP314711SZB
CSB-BP314711SZB
CSB-MP314711SZB
CSB-EP314711SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S1 (rpsA) CSB-YP314712EGX
CSB-EP314712EGX
CSB-BP314712EGX
CSB-MP314712EGX
CSB-EP314712EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant sn-glycerol-3-phosphate-binding periplasmic protein ugpB (ugpB) CSB-YP314713EOD
CSB-EP314713EOD
CSB-BP314713EOD
CSB-MP314713EOD
CSB-EP314713EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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