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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Transposase for insertion sequence element IS1151 (tnp) CSB-YP313163CMB
CSB-EP313163CMB
CSB-BP313163CMB
CSB-MP313163CMB
CSB-EP313163CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial CSB-YP313164ZAB
CSB-BP313164ZAB
CSB-MP313164ZAB
CSB-EP313164ZAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Pyruvate decarboxylase isozyme 3 (PDC3) CSB-YP313165ZAX
CSB-EP313165ZAX
CSB-BP313165ZAX
CSB-MP313165ZAX
CSB-EP313165ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3,4-dihydroxyphenylacetate 2,3-dioxygenase (hpcB) CSB-YP313166ENL
CSB-EP313166ENL
CSB-BP313166ENL
CSB-MP313166ENL
CSB-EP313166ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein ERP1 (ERP1), partial CSB-YP313167SVG
CSB-EP313167SVG
CSB-BP313167SVG
CSB-MP313167SVG
CSB-EP313167SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable acyl carrier protein (sch3) CSB-YP313168SON
CSB-EP313168SON
CSB-BP313168SON
CSB-MP313168SON
CSB-EP313168SON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative polyketide cyclase (sch4) CSB-YP313169SON
CSB-EP313169SON
CSB-BP313169SON
CSB-MP313169SON
CSB-EP313169SON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase gamma chain (atpG) CSB-YP313170SSI
CSB-EP313170SSI
CSB-BP313170SSI
CSB-MP313170SSI
CSB-EP313170SSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP313171LET
CSB-EP313171LET
CSB-BP313171LET
CSB-MP313171LET
CSB-EP313171LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Uridylate kinase (pyrH) CSB-YP313172LET
CSB-EP313172LET
CSB-BP313172LET
CSB-MP313172LET
CSB-EP313172LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Dihydrodipicolinate synthase (dapA) CSB-YP313173LET
CSB-EP313173LET
CSB-BP313173LET
CSB-MP313173LET
CSB-EP313173LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana L-ascorbate peroxidase 1, cytosolic (APX1) CSB-YP313174DOA
CSB-EP313174DOA
CSB-BP313174DOA
CSB-MP313174DOA
CSB-EP313174DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ubiquitin-like protein FUBI (Fau) CSB-YP313175RA
CSB-EP313175RA
CSB-BP313175RA
CSB-MP313175RA
CSB-EP313175RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype F2 Protein P (P), partial CSB-YP313176HEP
CSB-EP313176HEP
CSB-BP313176HEP
CSB-MP313176HEP
CSB-EP313176HEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera berteriana 60S ribosomal protein L5, mitochondrial (RPL5) CSB-YP313177OAR
CSB-EP313177OAR
CSB-BP313177OAR
CSB-MP313177OAR
CSB-EP313177OAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 30S ribosomal protein S12 (rpsL) CSB-YP313178LET
CSB-EP313178LET
CSB-BP313178LET
CSB-MP313178LET
CSB-EP313178LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L1 (rplA) CSB-YP313179LET
CSB-EP313179LET
CSB-BP313179LET
CSB-MP313179LET
CSB-EP313179LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP313180LET
CSB-EP313180LET
CSB-BP313180LET
CSB-MP313180LET
CSB-EP313180LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Methionine--tRNA ligase (metG), partial CSB-YP313181LET
CSB-EP313181LET
CSB-BP313181LET
CSB-MP313181LET
CSB-EP313181LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Arginine--tRNA ligase, cytoplasmic (YDR341C), partial CSB-YP313182SVG
CSB-EP313182SVG
CSB-BP313182SVG
CSB-MP313182SVG
CSB-EP313182SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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