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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chenopodium rubrum 18.3 kDa class I heat shock protein (HSP18) CSB-YP313224DRT
CSB-EP313224DRT
CSB-BP313224DRT
CSB-MP313224DRT
CSB-EP313224DRT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Peroxidase CSB-YP313225TQN
CSB-EP313225TQN
CSB-BP313225TQN
CSB-MP313225TQN
CSB-EP313225TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Formin (LD), partial CSB-YP313226CH
CSB-EP313226CH
CSB-BP313226CH
CSB-MP313226CH
CSB-EP313226CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum 78 kDa glucose-regulated protein homolog, partial CSB-YP313227PMB
CSB-EP313227PMB
CSB-BP313227PMB
CSB-MP313227PMB
CSB-EP313227PMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 3,2-trans-enoyl-CoA isomerase (ECI1) CSB-YP313228SVG
CSB-EP313228SVG
CSB-BP313228SVG
CSB-MP313228SVG
CSB-EP313228SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Hepatocellular carcinoma protein HHCM CSB-YP313229HU
CSB-EP313229HU
CSB-BP313229HU
CSB-MP313229HU
CSB-EP313229HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galactofuranosyl glycosyltransferase (LPG1), partial CSB-YP313230LOE
CSB-EP313230LOE
CSB-BP313230LOE
CSB-MP313230LOE
CSB-EP313230LOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis armigera entomopoxvirus Spindolin CSB-YP313231HVE
CSB-EP313231HVE
CSB-BP313231HVE
CSB-MP313231HVE
CSB-EP313231HVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri Chorismate synthase (aroC) CSB-YP313232BNA
CSB-EP313232BNA
CSB-BP313232BNA
CSB-MP313232BNA
CSB-EP313232BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri Glutamate--tRNA ligase (gltX) CSB-YP313233BNA
CSB-EP313233BNA
CSB-BP313233BNA
CSB-MP313233BNA
CSB-EP313233BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri 10 kDa chaperonin (groS) CSB-YP313234BNA
CSB-EP313234BNA
CSB-BP313234BNA
CSB-MP313234BNA
CSB-EP313234BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP313235BNA
CSB-EP313235BNA
CSB-BP313235BNA
CSB-MP313235BNA
CSB-EP313235BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein HRI1 (HRI1) CSB-YP313236SVG
CSB-EP313236SVG
CSB-BP313236SVG
CSB-MP313236SVG
CSB-EP313236SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 2-5A-dependent ribonuclease (Rnasel), partial CSB-YP313237MO
CSB-EP313237MO
CSB-BP313237MO
CSB-MP313237MO
CSB-EP313237MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Heat shock protein SSQ1, mitochondrial (SSQ1), partial CSB-YP313238SVG
CSB-EP313238SVG
CSB-BP313238SVG
CSB-MP313238SVG
CSB-EP313238SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae F-box protein YLR224W (YLR224W) CSB-YP313239SVG
CSB-EP313239SVG
CSB-BP313239SVG
CSB-MP313239SVG
CSB-EP313239SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dianthus caryophyllus Petal death protein (PDP) CSB-YP313240DKA
CSB-EP313240DKA
CSB-BP313240DKA
CSB-MP313240DKA
CSB-EP313240DKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Matthiola incana Naringenin,2-oxoglutarate 3-dioxygenase (FHT) CSB-YP313241MQK
CSB-EP313241MQK
CSB-BP313241MQK
CSB-MP313241MQK
CSB-EP313241MQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 10 kDa chaperonin (groS) CSB-YP313242SSQ
CSB-EP313242SSQ
CSB-BP313242SSQ
CSB-MP313242SSQ
CSB-EP313242SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cercidiphyllum japonicum Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP313243CGQ
CSB-EP313243CGQ
CSB-BP313243CGQ
CSB-MP313243CGQ
CSB-EP313243CGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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