Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 74 (VPS74) CSB-YP313287SVG
CSB-EP313287SVG
CSB-BP313287SVG
CSB-MP313287SVG
CSB-EP313287SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DOCK-like protein YLR422W (YLR422W), partial CSB-YP313288SVG
CSB-EP313288SVG
CSB-BP313288SVG
CSB-MP313288SVG
CSB-EP313288SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized oxidoreductase TDA5 (TDA5), partial CSB-YP313289SVG1
CSB-EP313289SVG1
CSB-BP313289SVG1
CSB-MP313289SVG1
CSB-EP313289SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-dependent glutamate synthase (gltB), partial CSB-YP313291ALT
CSB-EP313291ALT
CSB-BP313291ALT
CSB-MP313291ALT
CSB-EP313291ALT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrate reductase (nasA), partial CSB-YP313292KBE
CSB-EP313292KBE
CSB-BP313292KBE
CSB-MP313292KBE
CSB-EP313292KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Protein psbN (psbN), partial CSB-YP313293DSJ
CSB-EP313293DSJ
CSB-BP313293DSJ
CSB-MP313293DSJ
CSB-EP313293DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative pyridoxal reductase (YPR127W) CSB-YP313294SVG
CSB-EP313294SVG
CSB-BP313294SVG
CSB-MP313294SVG
CSB-EP313294SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nucleolar complex protein 4 (NOC4), partial CSB-YP313295SVG
CSB-EP313295SVG
CSB-BP313295SVG
CSB-MP313295SVG
CSB-EP313295SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allochromatium vinosum Ankyrin homolog (Alvin_1094) CSB-YP313296AZP
CSB-EP313296AZP
CSB-BP313296AZP
CSB-MP313296AZP
CSB-EP313296AZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein kinase APK1A, chloroplastic (APK1A) CSB-YP313297DOA
CSB-EP313297DOA
CSB-BP313297DOA
CSB-MP313297DOA
CSB-EP313297DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Palmitoyltransferase ERF2 (ERF2), partial CSB-YP313298SVG1
CSB-EP313298SVG1
CSB-BP313298SVG1
CSB-MP313298SVG1
CSB-EP313298SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Basement membrane proteoglycan (unc-52), partial CSB-YP313299CXY
CSB-EP313299CXY
CSB-BP313299CXY
CSB-MP313299CXY
CSB-EP313299CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila psittaci Early upstream open reading frame (CPSIT_0205, G5O_0207) CSB-YP313300DSM
CSB-EP313300DSM
CSB-BP313300DSM
CSB-MP313300DSM
CSB-EP313300DSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Pyrophosphate-energized vacuolar membrane proton pump, partial CSB-YP313301HWQ
CSB-EP313301HWQ
CSB-BP313301HWQ
CSB-MP313301HWQ
CSB-EP313301HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tamias sibiricus Hibernation-associated plasma protein HP-27 CSB-YP313302TLD
CSB-EP313302TLD
CSB-BP313302TLD
CSB-MP313302TLD
CSB-EP313302TLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyocin-S1 (pys1), partial CSB-YP313303EZW
CSB-EP313303EZW
CSB-BP313303EZW
CSB-MP313303EZW
CSB-EP313303EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4) CSB-YP313304DOA
CSB-EP313304DOA
CSB-BP313304DOA
CSB-MP313304DOA
CSB-EP313304DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YPR195C (YPR195C) CSB-YP313305SVG
CSB-EP313305SVG
CSB-BP313305SVG
CSB-MP313305SVG
CSB-EP313305SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Granzyme-like protein 2 (Mcpt10) CSB-YP313306RA
CSB-EP313306RA
CSB-BP313306RA
CSB-MP313306RA
CSB-EP313306RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans RuBisCO operon transcriptional regulator (rbcR) CSB-YP313307TOA
CSB-EP313307TOA
CSB-BP313307TOA
CSB-MP313307TOA
CSB-EP313307TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>