Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica 50S ribosomal protein L2, chloroplastic (rpl2-A) CSB-YP313940OFG
CSB-EP313940OFG
CSB-BP313940OFG
CSB-MP313940OFG
CSB-EP313940OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP313941OCO
CSB-EP313941OCO
CSB-BP313941OCO
CSB-MP313941OCO
CSB-EP313941OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP313943OFF
CSB-EP313943OFF
CSB-BP313943OFF
CSB-MP313943OFF
CSB-EP313943OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP313944OCO
CSB-EP313944OCO
CSB-BP313944OCO
CSB-MP313944OCO
CSB-EP313944OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica ATP synthase subunit 9, mitochondrial (ATP9), partial CSB-YP313945OFF1
CSB-EP313945OFF1
CSB-BP313945OFF1
CSB-MP313945OFF1
CSB-EP313945OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Type IV secretion system protein virB4 (virB4), partial CSB-YP313946BWS
CSB-EP313946BWS
CSB-BP313946BWS
CSB-MP313946BWS
CSB-EP313946BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Beta- (1-->2)glucan export ATP-binding/permease protein NdvA, partial CSB-YP313947BWS1
CSB-EP313947BWS1
CSB-BP313947BWS1
CSB-MP313947BWS1
CSB-EP313947BWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Type IV secretion system putative lipoprotein virB7 (virB7) CSB-YP313948BWS
CSB-EP313948BWS
CSB-BP313948BWS
CSB-MP313948BWS
CSB-EP313948BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Actin-7 (ACT7) CSB-YP313949OFG
CSB-EP313949OFG
CSB-BP313949OFG
CSB-MP313949OFG
CSB-EP313949OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus fasciatus Neurotoxin 3FTx-LI CSB-YP313950BXL
CSB-EP313950BXL
CSB-BP313950BXL
CSB-MP313950BXL
CSB-EP313950BXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus fasciatus Neurotoxin 3FTx-RI CSB-YP313951BXL
CSB-EP313951BXL
CSB-BP313951BXL
CSB-MP313951BXL
CSB-EP313951BXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA gyrase subunit B (gyrB), partial CSB-YP313952MVW
CSB-EP313952MVW
CSB-BP313952MVW
CSB-MP313952MVW
CSB-EP313952MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella choleraesuis Purine ribonucleoside efflux pump nepI (nepI), partial CSB-YP313953SBF
CSB-EP313953SBF
CSB-BP313953SBF
CSB-MP313953SBF
CSB-EP313953SBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine polyomavirus Small t antigen CSB-YP313954MKJ
CSB-EP313954MKJ
CSB-BP313954MKJ
CSB-MP313954MKJ
CSB-EP313954MKJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mokola virus Matrix protein (M) CSB-YP313955MHF
CSB-EP313955MHF
CSB-BP313955MHF
CSB-MP313955MHF
CSB-EP313955MHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M), partial CSB-YP313956ICQ1
CSB-EP313956ICQ1
CSB-BP313956ICQ1
CSB-MP313956ICQ1
CSB-EP313956ICQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Putative mitochondrial 2-oxoglutarate/malate carrier protein (NCU04792, NCU10732), partial CSB-YP313957NHA1
CSB-EP313957NHA1
CSB-BP313957NHA1
CSB-MP313957NHA1
CSB-EP313957NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Soluble starch synthase 2-3, chloroplastic/amyloplastic (SSII-3), partial CSB-YP313958OFF
CSB-EP313958OFF
CSB-BP313958OFF
CSB-MP313958OFF
CSB-EP313958OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius ussuriensis Thrombin-like enzyme calobin-2 CSB-YP313959GGS
CSB-EP313959GGS
CSB-BP313959GGS
CSB-MP313959GGS
CSB-EP313959GGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Late embryogenesis abundant protein, group 3 (LEA3) CSB-YP313960OFG
CSB-EP313960OFG
CSB-BP313960OFG
CSB-MP313960OFG
CSB-EP313960OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>