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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant African swine fever virus Uncharacterized protein E66L (Pret-148) CSB-YP315504AYG
CSB-EP315504AYG
CSB-BP315504AYG
CSB-MP315504AYG
CSB-EP315504AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus leopardus Alpha-conotoxin-like Lp1.6a CSB-YP315505CXD
CSB-EP315505CXD
CSB-BP315505CXD
CSB-MP315505CXD
CSB-EP315505CXD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cerastes cerastes Phospholipase A2 1 CSB-YP315506CGI
CSB-EP315506CGI
CSB-BP315506CGI
CSB-MP315506CGI
CSB-EP315506CGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus durissus terrificus Phospholipase A2 F17 CSB-YP315507DYD
CSB-EP315507DYD
CSB-BP315507DYD
CSB-MP315507DYD
CSB-EP315507DYD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus pyrrhocryptus Phospholipase A2 D6 CSB-YP315508MGP
CSB-EP315508MGP
CSB-BP315508MGP
CSB-MP315508MGP
CSB-EP315508MGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Alpha-ketoglutarate-dependent dioxygenase AlkB homolog (alkB) CSB-YP315509DQO
CSB-EP315509DQO
CSB-BP315509DQO
CSB-MP315509DQO
CSB-EP315509DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP315510DQO
CSB-EP315510DQO
CSB-BP315510DQO
CSB-MP315510DQO
CSB-EP315510DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Cell division protein FtsZ (ftsZ), partial CSB-YP315511DQO
CSB-EP315511DQO
CSB-BP315511DQO
CSB-MP315511DQO
CSB-EP315511DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus HTH-type transcriptional regulator skgA (skgA) CSB-YP315512DQO
CSB-EP315512DQO
CSB-BP315512DQO
CSB-MP315512DQO
CSB-EP315512DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Cell cycle transcriptional regulator CtrA (ctrA) CSB-YP315513DQO
CSB-EP315513DQO
CSB-BP315513DQO
CSB-MP315513DQO
CSB-EP315513DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative defensin-like protein 134 (At5g29576) CSB-YP315514DOA
CSB-EP315514DOA
CSB-BP315514DOA
CSB-MP315514DOA
CSB-EP315514DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachesana tarabaevi M-zodatoxin-Lt8k (cit 1-10) CSB-YP315515LAF
CSB-EP315515LAF
CSB-BP315515LAF
CSB-MP315515LAF
CSB-EP315515LAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachesana tarabaevi M-zodatoxin-Lt8p (cit 1-15) CSB-YP315516LAF
CSB-EP315516LAF
CSB-BP315516LAF
CSB-MP315516LAF
CSB-EP315516LAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyuranus scutellatus scutellatus Short neurotoxin 2 CSB-YP315517OGF
CSB-EP315517OGF
CSB-BP315517OGF
CSB-MP315517OGF
CSB-EP315517OGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus ammiralis Omega-conotoxin-like Ai6.3 CSB-YP315518CNU
CSB-EP315518CNU
CSB-BP315518CNU
CSB-MP315518CNU
CSB-EP315518CNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At3g50810 (At3g50810), partial CSB-YP315519DOA1
CSB-EP315519DOA1
CSB-BP315519DOA1
CSB-MP315519DOA1
CSB-EP315519DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g38065 (At4g38065) CSB-YP315520DOA
CSB-EP315520DOA
CSB-BP315520DOA
CSB-MP315520DOA
CSB-EP315520DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP315521SGE1
CSB-EP315521SGE1
CSB-BP315521SGE1
CSB-MP315521SGE1
CSB-EP315521SGE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Methylglyoxal synthase (mgsA) CSB-YP315522BWS
CSB-EP315522BWS
CSB-BP315522BWS
CSB-MP315522BWS
CSB-EP315522BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM203B (FAM203B) CSB-YP315523HU
CSB-EP315523HU
CSB-BP315523HU
CSB-MP315523HU
CSB-EP315523HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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