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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP315524SNE
CSB-EP315524SNE
CSB-BP315524SNE
CSB-MP315524SNE
CSB-EP315524SNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--D-alanine ligase (ddl) CSB-YP315525FMW
CSB-EP315525FMW
CSB-BP315525FMW
CSB-MP315525FMW
CSB-EP315525FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (NDUFA10) CSB-YP315526PYX
CSB-EP315526PYX
CSB-BP315526PYX
CSB-MP315526PYX
CSB-EP315526PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (NDUFS8) CSB-YP315527EXP
CSB-EP315527EXP
CSB-BP315527EXP
CSB-MP315527EXP
CSB-EP315527EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Large cysteine-rich periplasmic protein omcB (omcB), partial CSB-YP315528DSB
CSB-BP315528DSB
CSB-MP315528DSB
CSB-EP315528DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterometrus bengalensis Bengalin CSB-YP315529HGO
CSB-EP315529HGO
CSB-BP315529HGO
CSB-MP315529HGO
CSB-EP315529HGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lasiodora sp. U1-theraphotoxin-Lsp1a CSB-YP315530LLI
CSB-EP315530LLI
CSB-BP315530LLI
CSB-MP315530LLI
CSB-EP315530LLI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Alpha-glucosidase (malA), partial CSB-YP315531FPM
CSB-EP315531FPM
CSB-BP315531FPM
CSB-MP315531FPM
CSB-EP315531FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombus lapidarius Bombolitin-8 CSB-YP315532BID
CSB-EP315532BID
CSB-BP315532BID
CSB-MP315532BID
CSB-EP315532BID-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Phosphoglycerate kinase (pgk) CSB-YP315533DSB
CSB-EP315533DSB
CSB-BP315533DSB
CSB-MP315533DSB
CSB-EP315533DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis 50S ribosomal protein L16 (rplP) CSB-YP315534DSB
CSB-EP315534DSB
CSB-BP315534DSB
CSB-MP315534DSB
CSB-EP315534DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis 30S ribosomal protein S11 (rpsK) CSB-YP315535DSB
CSB-EP315535DSB
CSB-BP315535DSB
CSB-MP315535DSB
CSB-EP315535DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP315536DSB
CSB-EP315536DSB
CSB-BP315536DSB
CSB-MP315536DSB
CSB-EP315536DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Histone H1-like protein Hc1 (hctA) CSB-YP315537DSB
CSB-EP315537DSB
CSB-BP315537DSB
CSB-MP315537DSB
CSB-EP315537DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Pyruvate kinase (pyk) CSB-YP315538DSB
CSB-EP315538DSB
CSB-BP315538DSB
CSB-MP315538DSB
CSB-EP315538DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus capitaneus Alpha-conotoxin-like Cp20.4 CSB-YP315539DVV
CSB-EP315539DVV
CSB-BP315539DVV
CSB-MP315539DVV
CSB-EP315539DVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus mustelinus Alpha-conotoxin-like Ms20.5 CSB-YP315540DWF
CSB-EP315540DWF
CSB-BP315540DWF
CSB-MP315540DWF
CSB-EP315540DWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Elongation factor Tu 1 (tufA) CSB-YP315541ENV
CSB-EP315541ENV
CSB-BP315541ENV
CSB-MP315541ENV
CSB-EP315541ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insH for insertion sequence element IS5H (insH6) CSB-YP315542ENV
CSB-EP315542ENV
CSB-BP315542ENV
CSB-MP315542ENV
CSB-EP315542ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insH for insertion sequence element IS5T (insH11) CSB-YP315543ENV
CSB-EP315543ENV
CSB-BP315543ENV
CSB-MP315543ENV
CSB-EP315543ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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