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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Transposase insC for insertion element IS2-8 (JW5900) CSB-YP316010ENV
CSB-EP316010ENV
CSB-BP316010ENV
CSB-MP316010ENV
CSB-EP316010ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insD for insertion element IS2-10 (JW5903) CSB-YP316011ENV
CSB-EP316011ENV
CSB-BP316011ENV
CSB-MP316011ENV
CSB-EP316011ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insE for insertion sequence IS3fA (insE6) CSB-YP316012ENV
CSB-EP316012ENV
CSB-BP316012ENV
CSB-MP316012ENV
CSB-EP316012ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Endogenous Bornavirus-like nucleoprotein 1 (EBLN1) CSB-YP316013HU
CSB-EP316013HU
CSB-BP316013HU
CSB-MP316013HU
CSB-EP316013HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insF for insertion sequence IS3D (insF4) CSB-YP316014ENV
CSB-EP316014ENV
CSB-BP316014ENV
CSB-MP316014ENV
CSB-EP316014ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative arsenate reductase (yfjU) CSB-YP316015ENV
CSB-EP316015ENV
CSB-BP316015ENV
CSB-MP316015ENV
CSB-EP316015ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transposase InsL for insertion sequence element IS186C (insL3) CSB-YP316016ENV
CSB-EP316016ENV
CSB-BP316016ENV
CSB-MP316016ENV
CSB-EP316016ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops alternatus Thrombin-like enzyme bhalternin CSB-YP316017BUS
CSB-EP316017BUS
CSB-BP316017BUS
CSB-MP316017BUS
CSB-EP316017BUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NHL-repeat-containing protein 4 (NHLRC4) CSB-YP316018HU
CSB-EP316018HU
CSB-BP316018HU
CSB-MP316018HU
CSB-EP316018HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Flagellar motor switch protein FliM (fliM) CSB-YP316019DQO
CSB-EP316019DQO
CSB-BP316019DQO
CSB-MP316019DQO
CSB-EP316019DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human POTE ankyrin domain family member J (POTEJ), partial CSB-YP316020HU
CSB-EP316020HU
CSB-BP316020HU
CSB-MP316020HU
CSB-EP316020HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cutaneous T-cell lymphoma-associated antigen 8 (CTAGE8), partial CSB-YP316021HU1
CSB-EP316021HU1
CSB-BP316021HU1
CSB-MP316021HU1
CSB-EP316021HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Polyubiquitin-B (UBB) CSB-YP316022HU
CSB-EP316022HU
CSB-BP316022HU
CSB-MP316022HU
CSB-EP316022HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Polyubiquitin-C (UBC) CSB-YP316023EQV
CSB-EP316023EQV
CSB-BP316023EQV
CSB-MP316023EQV
CSB-EP316023EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Polyubiquitin-A (ubqA) CSB-YP316024DKK
CSB-EP316024DKK
CSB-BP316024DKK
CSB-MP316024DKK
CSB-EP316024DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Polyubiquitin-G (ubqG) CSB-YP316025DKK
CSB-EP316025DKK
CSB-BP316025DKK
CSB-MP316025DKK
CSB-EP316025DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana sylvestris Polyubiquitin (UBI4) CSB-YP316026NHD
CSB-EP316026NHD
CSB-BP316026NHD
CSB-MP316026NHD
CSB-EP316026NHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv3118 (Rv3118) CSB-YP316027MVZ
CSB-EP316027MVZ
CSB-BP316027MVZ
CSB-MP316027MVZ
CSB-EP316027MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative RING-H2 finger protein ATL21A (ATL21A), partial CSB-YP316028DOA1
CSB-EP316028DOA1
CSB-BP316028DOA1
CSB-MP316028DOA1
CSB-EP316028DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus parius Conotoxin pr3a CSB-YP316029COZ
CSB-EP316029COZ
CSB-BP316029COZ
CSB-MP316029COZ
CSB-EP316029COZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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