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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Acyl-coenzyme A oxidase 3, peroxisomal (ACX3), partial CSB-YP316979DOA
CSB-EP316979DOA
CSB-BP316979DOA
CSB-MP316979DOA
CSB-EP316979DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP316980SMW
CSB-EP316980SMW
CSB-BP316980SMW
CSB-MP316980SMW
CSB-EP316980SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP316981SMW
CSB-EP316981SMW
CSB-BP316981SMW
CSB-MP316981SMW
CSB-EP316981SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP316982SMW
CSB-EP316982SMW
CSB-BP316982SMW
CSB-MP316982SMW
CSB-EP316982SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetate kinase (ackA) CSB-YP316983SMV
CSB-EP316983SMV
CSB-BP316983SMV
CSB-MP316983SMV
CSB-EP316983SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine racemase (alr) CSB-YP316984SMV
CSB-EP316984SMV
CSB-BP316984SMV
CSB-MP316984SMV
CSB-EP316984SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Fructose-bisphosphate aldolase (fba) CSB-YP316985SMW
CSB-EP316985SMW
CSB-BP316985SMW
CSB-MP316985SMW
CSB-EP316985SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine deiminase (arcA) CSB-YP316986SMV
CSB-EP316986SMV
CSB-BP316986SMV
CSB-MP316986SMV
CSB-EP316986SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine repressor (argR2) CSB-YP316987SMV
CSB-EP316987SMV
CSB-BP316987SMV
CSB-MP316987SMV
CSB-EP316987SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 3-dehydroquinate dehydratase (aroD) CSB-YP316988SMW
CSB-EP316988SMW
CSB-BP316988SMW
CSB-MP316988SMW
CSB-EP316988SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Chorismate synthase (aroC) CSB-YP316989SMW
CSB-EP316989SMW
CSB-BP316989SMW
CSB-MP316989SMW
CSB-EP316989SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate--ammonia ligase (asnA) CSB-YP316990SMV
CSB-EP316990SMV
CSB-BP316990SMV
CSB-MP316990SMV
CSB-EP316990SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Aspartate--ammonia ligase (asnA) CSB-YP316991SMW
CSB-EP316991SMW
CSB-BP316991SMW
CSB-MP316991SMW
CSB-EP316991SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 ATP synthase epsilon chain (atpC) CSB-YP316992SMW
CSB-EP316992SMW
CSB-BP316992SMW
CSB-MP316992SMW
CSB-EP316992SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant V-type ATP synthase alpha chain (atpA), partial CSB-YP316993SMV
CSB-EP316993SMV
CSB-BP316993SMV
CSB-MP316993SMV
CSB-EP316993SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 V-type ATP synthase beta chain (atpB) CSB-YP316994SMW
CSB-EP316994SMW
CSB-BP316994SMW
CSB-MP316994SMW
CSB-EP316994SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP316995SMW
CSB-EP316995SMW
CSB-BP316995SMW
CSB-MP316995SMW
CSB-EP316995SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP316996SMV
CSB-EP316996SMV
CSB-BP316996SMV
CSB-MP316996SMV
CSB-EP316996SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Putative competence-damage inducible protein (cinA) CSB-YP316997SMW
CSB-EP316997SMW
CSB-BP316997SMW
CSB-MP316997SMW
CSB-EP316997SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP316998SMV
CSB-EP316998SMV
CSB-BP316998SMV
CSB-MP316998SMV
CSB-EP316998SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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