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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Orotate phosphoribosyltransferase (pyrE) CSB-YP316110PXQ
CSB-EP316110PXQ
CSB-BP316110PXQ
CSB-MP316110PXQ
CSB-EP316110PXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Iron-sulfur clusters transporter ATM1, mitochondrial (ATM1), partial CSB-YP316111CTK1
CSB-EP316111CTK1
CSB-BP316111CTK1
CSB-MP316111CTK1
CSB-EP316111CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-rRNA-processing protein ESF2 (ESF2) CSB-YP316112CTK
CSB-EP316112CTK
CSB-BP316112CTK
CSB-MP316112CTK
CSB-EP316112CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Homoaconitase, mitochondrial (LYS4), partial CSB-YP316113CTL
CSB-EP316113CTL
CSB-BP316113CTL
CSB-MP316113CTL
CSB-EP316113CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cofilin (COF1) CSB-YP316114CTK
CSB-EP316114CTK
CSB-BP316114CTK
CSB-MP316114CTK
CSB-EP316114CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP316115CTK
CSB-EP316115CTK
CSB-BP316115CTK
CSB-MP316115CTK
CSB-EP316115CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (CNBD2760), partial CSB-YP316116CTK
CSB-EP316116CTK
CSB-BP316116CTK
CSB-MP316116CTK
CSB-EP316116CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 9 (ATG9), partial CSB-YP316117CTK
CSB-EP316117CTK
CSB-BP316117CTK
CSB-MP316117CTK
CSB-EP316117CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein BTN1 (BTN1), partial CSB-YP316118CTK
CSB-EP316118CTK
CSB-BP316118CTK
CSB-MP316118CTK
CSB-EP316118CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D pH-response regulator protein palA/RIM20 (RIM20), partial CSB-YP316119CTK
CSB-EP316119CTK
CSB-BP316119CTK
CSB-MP316119CTK
CSB-EP316119CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein CGI121 (CGI121) CSB-YP316120CTL
CSB-EP316120CTL
CSB-BP316120CTL
CSB-MP316120CTL
CSB-EP316120CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Chitin synthase export chaperone (CHS7), partial CSB-YP316121CTL1
CSB-EP316121CTL1
CSB-BP316121CTL1
CSB-MP316121CTL1
CSB-EP316121CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein STU1 (STU1), partial CSB-YP316122CTL
CSB-EP316122CTL
CSB-BP316122CTL
CSB-MP316122CTL
CSB-EP316122CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein CLP1 (CLP1), partial CSB-YP316123CTK
CSB-EP316123CTK
CSB-BP316123CTK
CSB-MP316123CTK
CSB-EP316123CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Endoribonuclease YSH1 (YSH1), partial CSB-YP316124CTL
CSB-EP316124CTL
CSB-BP316124CTL
CSB-MP316124CTL
CSB-EP316124CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein PNS1 (PNS1), partial CSB-YP316125CTK
CSB-EP316125CTK
CSB-BP316125CTK
CSB-MP316125CTK
CSB-EP316125CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CWC21 (CWC21) CSB-YP316126CTK
CSB-EP316126CTK
CSB-BP316126CTK
CSB-MP316126CTK
CSB-EP316126CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CWC25 (CWC25) CSB-YP316127CTK
CSB-EP316127CTK
CSB-BP316127CTK
CSB-MP316127CTK
CSB-EP316127CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Sulfate adenylyltransferase (MET3), partial CSB-YP316128CTL
CSB-EP316128CTL
CSB-BP316128CTL
CSB-MP316128CTL
CSB-EP316128CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein YOP1 (YOP1), partial CSB-YP316129CTL1
CSB-EP316129CTL1
CSB-BP316129CTL1
CSB-MP316129CTL1
CSB-EP316129CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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