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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D rRNA-processing protein EFG1 (EFG1) CSB-YP316130CTK
CSB-EP316130CTK
CSB-BP316130CTK
CSB-MP316130CTK
CSB-EP316130CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Exocyst complex component EXO84 (EXO84), partial CSB-YP316131CTL
CSB-EP316131CTL
CSB-BP316131CTL
CSB-MP316131CTL
CSB-EP316131CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Hsp70 nucleotide exchange factor FES1 (FES1) CSB-YP316132CTL
CSB-EP316132CTL
CSB-BP316132CTL
CSB-MP316132CTL
CSB-EP316132CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Endoplasmic reticulum vesicle protein 25 (ERV25), partial CSB-YP316133CTK
CSB-EP316133CTK
CSB-BP316133CTK
CSB-MP316133CTK
CSB-EP316133CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D UDP-N-acetylglucosamine transferase subunit ALG13 (ALG13) CSB-YP316134CTL
CSB-EP316134CTL
CSB-BP316134CTL
CSB-MP316134CTL
CSB-EP316134CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1) CSB-YP316135CTL
CSB-EP316135CTL
CSB-BP316135CTL
CSB-MP316135CTL
CSB-EP316135CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CLF1 (CLF1), partial CSB-YP316136CTK
CSB-EP316136CTK
CSB-BP316136CTK
CSB-MP316136CTK
CSB-EP316136CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Helicase SWR1 (SWR1), partial CSB-YP316137CTL
CSB-EP316137CTL
CSB-BP316137CTL
CSB-MP316137CTL
CSB-EP316137CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D JmjC domain-containing histone demethylation protein 1 (JHD1), partial CSB-YP316138CTL
CSB-EP316138CTL
CSB-BP316138CTL
CSB-MP316138CTL
CSB-EP316138CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 8 (ATG8) CSB-YP316139CTK
CSB-EP316139CTK
CSB-BP316139CTK
CSB-MP316139CTK
CSB-EP316139CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Putative lipase ATG15 (ATG15), partial CSB-YP316140CTK
CSB-EP316140CTK
CSB-BP316140CTK
CSB-MP316140CTK
CSB-EP316140CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Vacuolar fusion protein MON1 (MON1), partial CSB-YP316141CTL
CSB-EP316141CTL
CSB-BP316141CTL
CSB-MP316141CTL
CSB-EP316141CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cytosolic Fe-S cluster assembly factor CFD1 (CFD1) CSB-YP316142CTL
CSB-EP316142CTL
CSB-BP316142CTL
CSB-MP316142CTL
CSB-EP316142CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CEF1 (CEF1), partial CSB-YP316143CTL
CSB-EP316143CTL
CSB-BP316143CTL
CSB-MP316143CTL
CSB-EP316143CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Chromatin modification-related protein EAF1 (EAF1), partial CSB-YP316144CTK
CSB-EP316144CTK
CSB-BP316144CTK
CSB-MP316144CTK
CSB-EP316144CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cytosolic Fe-S cluster assembly factor NAR1 (NAR1), partial CSB-YP316145CTK
CSB-EP316145CTK
CSB-BP316145CTK
CSB-MP316145CTK
CSB-EP316145CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Probable kinetochore protein NDC80 (NDC80), partial CSB-YP316146CTK
CSB-EP316146CTK
CSB-BP316146CTK
CSB-MP316146CTK
CSB-EP316146CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Clampless protein 1 (CNBB4530) CSB-YP316147CTK
CSB-EP316147CTK
CSB-BP316147CTK
CSB-MP316147CTK
CSB-EP316147CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D UDP-galactose transporter homolog 1 (HUT1-A), partial CSB-YP316148CTL
CSB-EP316148CTL
CSB-BP316148CTL
CSB-MP316148CTL
CSB-EP316148CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Putative nicotinamide N-methyltransferase (NNT1) CSB-YP316149CTL
CSB-EP316149CTL
CSB-BP316149CTL
CSB-MP316149CTL
CSB-EP316149CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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