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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial intermediate peptidase 1 (OCT1), partial CSB-YP317322CTL
CSB-EP317322CTL
CSB-BP317322CTL
CSB-MP317322CTL
CSB-EP317322CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Phosphoribosylaminoimidazole carboxylase (ADE2), partial CSB-YP317323CTK
CSB-EP317323CTK
CSB-BP317323CTK
CSB-MP317323CTK
CSB-EP317323CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Ras-like protein (RAS1) CSB-YP317324CTK
CSB-EP317324CTK
CSB-BP317324CTK
CSB-MP317324CTK
CSB-EP317324CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein SEY1 (SEY1), partial CSB-YP317325CTL
CSB-EP317325CTL
CSB-BP317325CTL
CSB-MP317325CTL
CSB-EP317325CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 60S ribosomal protein L44 (RPL44) CSB-YP317326CTK
CSB-EP317326CTK
CSB-BP317326CTK
CSB-MP317326CTK
CSB-EP317326CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 37S ribosomal protein S10, mitochondrial (RSM10) CSB-YP317327CTL
CSB-EP317327CTL
CSB-BP317327CTL
CSB-MP317327CTL
CSB-EP317327CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 40S ribosomal protein S0 (RPS0) CSB-YP317328CTK
CSB-EP317328CTK
CSB-BP317328CTK
CSB-MP317328CTK
CSB-EP317328CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D E3 ubiquitin-protein ligase BRE1 (BRE1), partial CSB-YP317329CTL
CSB-EP317329CTL
CSB-BP317329CTL
CSB-MP317329CTL
CSB-EP317329CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D DNA repair protein RAD5 (RAD5), partial CSB-YP317330CTK
CSB-EP317330CTK
CSB-BP317330CTK
CSB-MP317330CTK
CSB-EP317330CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase FAL1 (FAL1) CSB-YP317331CTK
CSB-EP317331CTK
CSB-BP317331CTK
CSB-MP317331CTK
CSB-EP317331CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DBP3 (DBP3), partial CSB-YP317332CTL
CSB-EP317332CTL
CSB-BP317332CTL
CSB-MP317332CTL
CSB-EP317332CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DHH1 (DHH1), partial CSB-YP317333CTK
CSB-EP317333CTK
CSB-BP317333CTK
CSB-MP317333CTK
CSB-EP317333CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DBP5 (DBP5), partial CSB-YP317334CTK
CSB-EP317334CTK
CSB-BP317334CTK
CSB-MP317334CTK
CSB-EP317334CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DRS1 (DRS1), partial CSB-YP317335CTL
CSB-EP317335CTL
CSB-BP317335CTL
CSB-MP317335CTL
CSB-EP317335CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-processing ATP-dependent RNA helicase PRP5 (PRP5), partial CSB-YP317336CTL
CSB-EP317336CTL
CSB-BP317336CTL
CSB-MP317336CTL
CSB-EP317336CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase DBP8 (DBP8), partial CSB-YP317337CTK
CSB-EP317337CTK
CSB-BP317337CTK
CSB-MP317337CTK
CSB-EP317337CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent rRNA helicase SPB4 (SPB4), partial CSB-YP317338CTL
CSB-EP317338CTL
CSB-BP317338CTL
CSB-MP317338CTL
CSB-EP317338CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D ATP-dependent RNA helicase MRH4, mitochondrial (MRH4), partial CSB-YP317339CTK
CSB-EP317339CTK
CSB-BP317339CTK
CSB-MP317339CTK
CSB-EP317339CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor RSE1 (RSE1), partial CSB-YP317340CTL
CSB-EP317340CTL
CSB-BP317340CTL
CSB-MP317340CTL
CSB-EP317340CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D RuvB-like helicase 2 (RVB2) CSB-YP317341CTL
CSB-EP317341CTL
CSB-BP317341CTL
CSB-MP317341CTL
CSB-EP317341CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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