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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial import inner membrane translocase subunit TIM54 (TIM54), partial CSB-YP316190CTL1
CSB-EP316190CTL1
CSB-BP316190CTL1
CSB-MP316190CTL1
CSB-EP316190CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial import inner membrane translocase subunit TIM8 (TIM8) CSB-YP316191CTL
CSB-EP316191CTL
CSB-BP316191CTL
CSB-MP316191CTL
CSB-EP316191CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D GDP-mannose transporter 2 (GMT2), partial CSB-YP316192CTK
CSB-EP316192CTK
CSB-BP316192CTK
CSB-MP316192CTK
CSB-EP316192CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D tRNA (guanine (9)-N1)-methyltransferase CSB-YP316193CTK
CSB-EP316193CTK
CSB-BP316193CTK
CSB-MP316193CTK
CSB-EP316193CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D DNA damage-inducible protein 1 (DDI1) CSB-YP316194CTK
CSB-EP316194CTK
CSB-BP316194CTK
CSB-MP316194CTK
CSB-EP316194CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Plasma membrane proteolipid 3 (PMP3), partial CSB-YP316195CTL
CSB-EP316195CTL
CSB-BP316195CTL
CSB-MP316195CTL
CSB-EP316195CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Urease (URE1), partial CSB-YP316196CTK
CSB-EP316196CTK
CSB-BP316196CTK
CSB-MP316196CTK
CSB-EP316196CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Vacuolar protein sorting-associated protein 27 (VPS27), partial CSB-YP316197CTL
CSB-EP316197CTL
CSB-BP316197CTL
CSB-MP316197CTL
CSB-EP316197CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Probable cytosolic iron-sulfur protein assembly protein 1 (CIA1) CSB-YP316198CTL
CSB-EP316198CTL
CSB-BP316198CTL
CSB-MP316198CTL
CSB-EP316198CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone acetyltransferase type B subunit 2 (HAT2) CSB-YP316199CTK
CSB-EP316199CTK
CSB-BP316199CTK
CSB-MP316199CTK
CSB-EP316199CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial division protein 1 (MDV1), partial CSB-YP316200CTK
CSB-EP316200CTK
CSB-BP316200CTK
CSB-MP316200CTK
CSB-EP316200CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein transport protein SEC13 (SEC13) CSB-YP316201CTL
CSB-EP316201CTL
CSB-BP316201CTL
CSB-MP316201CTL
CSB-EP316201CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Ribosome biogenesis protein YTM1 (YTM1) CSB-YP316202CTK
CSB-EP316202CTK
CSB-BP316202CTK
CSB-MP316202CTK
CSB-EP316202CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Palmitoyltransferase AKR1 (AKR1), partial CSB-YP316203CTL1
CSB-EP316203CTL1
CSB-BP316203CTL1
CSB-MP316203CTL1
CSB-EP316203CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D AdoMet-dependent rRNA methyltransferase SPB1 (SPB1), partial CSB-YP316204CTL
CSB-EP316204CTL
CSB-BP316204CTL
CSB-MP316204CTL
CSB-EP316204CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Candidapepsin-2 (SAP2) CSB-BP316205CZF
CSB-MP316205CZF
CSB-EP316205CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe UPF0494 membrane protein CPT2R1.01c (SPBCPT2R1.01c, SPBPB2B2.20c), partial CSB-YP316206SXV1
CSB-EP316206SXV1
CSB-BP316206SXV1
CSB-MP316206SXV1
CSB-EP316206SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox C-type natriuretic peptide CSB-YP316207DYC
CSB-EP316207DYC
CSB-BP316207DYC
CSB-MP316207DYC
CSB-EP316207DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Acetate kinase (ackA) CSB-YP316208MSN
CSB-EP316208MSN
CSB-BP316208MSN
CSB-MP316208MSN
CSB-EP316208MSN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Protein grpE (grpE) CSB-YP316209MFC
CSB-EP316209MFC
CSB-BP316209MFC
CSB-MP316209MFC
CSB-EP316209MFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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