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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosynechococcus elongatus Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP317486TDQ1
CSB-EP317486TDQ1
CSB-BP317486TDQ1
CSB-MP317486TDQ1
CSB-EP317486TDQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Cyanophycinase (cphB) CSB-YP317487SSC
CSB-EP317487SSC
CSB-BP317487SSC
CSB-MP317487SSC
CSB-EP317487SSC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Cytosolic Fe-S cluster assembly factor cfd1 (cfd1) CSB-YP317488EKF
CSB-EP317488EKF
CSB-BP317488EKF
CSB-MP317488EKF
CSB-EP317488EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At5g08310, mitochondrial (At5g08310), partial CSB-YP317489DOA
CSB-EP317489DOA
CSB-BP317489DOA
CSB-MP317489DOA
CSB-EP317489DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laticauda colubrina Toxin Lc b CSB-YP317490LCZ
CSB-EP317490LCZ
CSB-BP317490LCZ
CSB-MP317490LCZ
CSB-EP317490LCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana hosii Brevinin-2HSa CSB-YP317491RAZ
CSB-EP317491RAZ
CSB-BP317491RAZ
CSB-MP317491RAZ
CSB-EP317491RAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana picturata Brevinin-2PTb CSB-YP317492RBD
CSB-EP317492RBD
CSB-BP317492RBD
CSB-MP317492RBD
CSB-EP317492RBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus monachus Alpha-conotoxin-like Mn1.4 CSB-YP317493COT
CSB-EP317493COT
CSB-BP317493COT
CSB-MP317493COT
CSB-EP317493COT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus striolatus Mu-conotoxin SxIIIA CSB-YP317494CPH
CSB-EP317494CPH
CSB-BP317494CPH
CSB-MP317494CPH
CSB-EP317494CPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus achatinus Omega-conotoxin-like Ac6.4 CSB-YP317495DVS
CSB-EP317495DVS
CSB-BP317495DVS
CSB-MP317495DVS
CSB-EP317495DVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadrurus gertschi Hg-scorpine-like 2 CSB-YP317496HST
CSB-EP317496HST
CSB-BP317496HST
CSB-MP317496HST
CSB-EP317496HST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus stigmurus Peptide 2699 CSB-YP317497TEV
CSB-EP317497TEV
CSB-BP317497TEV
CSB-MP317497TEV
CSB-EP317497TEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus stigmurus Toxin Tst3 CSB-YP317498TEV
CSB-EP317498TEV
CSB-BP317498TEV
CSB-MP317498TEV
CSB-EP317498TEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadrurus gertschi Potassium channel toxin alpha-KTx CSB-YP317499HST
CSB-EP317499HST
CSB-BP317499HST
CSB-MP317499HST
CSB-EP317499HST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Proteasome subunit alpha type-4-2 (OsI_021067, OsI_21826) CSB-YP317500OFF
CSB-EP317500OFF
CSB-BP317500OFF
CSB-MP317500OFF
CSB-EP317500OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus leopardus O1-superfamily conotoxin Leo-O2 CSB-YP317501CXD
CSB-EP317501CXD
CSB-BP317501CXD
CSB-MP317501CXD
CSB-EP317501CXD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Odontobuthus doriae Potassium channel toxin alpha-KTx 3.11 CSB-YP317502OAL
CSB-EP317502OAL
CSB-BP317502OAL
CSB-MP317502OAL
CSB-EP317502OAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Androctonus mauretanicus mauretanicus Alpha-toxin CSB-YP317503AKB
CSB-EP317503AKB
CSB-BP317503AKB
CSB-MP317503AKB
CSB-EP317503AKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Overexpressed in colon carcinoma 1 protein homolog CSB-YP317504BO
CSB-EP317504BO
CSB-BP317504BO
CSB-MP317504BO
CSB-EP317504BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans Ribulose bisphosphate carboxylase large chain 2 (cbbL2) CSB-YP317505TOA
CSB-EP317505TOA
CSB-BP317505TOA
CSB-MP317505TOA
CSB-EP317505TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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