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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptomycin resistance protein (str) CSB-YP318921FKZ
CSB-EP318921FKZ
CSB-BP318921FKZ
CSB-MP318921FKZ
CSB-EP318921FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Relaxosome protein TraY (traY) CSB-YP318922SWW
CSB-EP318922SWW
CSB-BP318922SWW
CSB-MP318922SWW
CSB-EP318922SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Tyrosine--tRNA ligase, mitochondrial (cyt-18), partial CSB-YP318923NHA
CSB-EP318923NHA
CSB-BP318923NHA
CSB-MP318923NHA
CSB-EP318923NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Lysozyme C-1 CSB-YP318924PI
CSB-EP318924PI
CSB-BP318924PI
CSB-MP318924PI
CSB-EP318924PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Integrin alpha-PS2 (if), partial CSB-YP318925DLU
CSB-EP318925DLU
CSB-BP318925DLU
CSB-MP318925DLU
CSB-EP318925DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP318926NEK1
CSB-EP318926NEK1
CSB-BP318926NEK1
CSB-MP318926NEK1
CSB-EP318926NEK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 30S ribosomal protein S16, chloroplastic (rps16) CSB-YP318927OFG
CSB-EP318927OFG
CSB-BP318927OFG
CSB-MP318927OFG
CSB-EP318927OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem II reaction center protein K (psbK) CSB-YP318928NHE
CSB-EP318928NHE
CSB-BP318928NHE
CSB-MP318928NHE
CSB-EP318928NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cytochrome b6-f complex subunit 6 (petL), partial CSB-YP318929OFG
CSB-EP318929OFG
CSB-BP318929OFG
CSB-MP318929OFG
CSB-EP318929OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Photosystem II reaction center protein J (psbJ), partial CSB-YP318930MCB
CSB-EP318930MCB
CSB-BP318930MCB
CSB-MP318930MCB
CSB-EP318930MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Photosystem II reaction center protein Z (psbZ), partial CSB-YP318931OFG1
CSB-EP318931OFG1
CSB-BP318931OFG1
CSB-MP318931OFG1
CSB-EP318931OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP318932NHE1
CSB-EP318932NHE1
CSB-BP318932NHE1
CSB-MP318932NHE1
CSB-EP318932NHE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Chloroplast envelope membrane protein (cemA), partial CSB-YP318933NHE1
CSB-EP318933NHE1
CSB-BP318933NHE1
CSB-MP318933NHE1
CSB-EP318933NHE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD) CSB-YP318934MCB
CSB-EP318934MCB
CSB-BP318934MCB
CSB-MP318934MCB
CSB-EP318934MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein L (psbL), partial CSB-YP318935EHU
CSB-EP318935EHU
CSB-BP318935EHU
CSB-MP318935EHU
CSB-EP318935EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Lichenase-2 CSB-YP318937HWQ
CSB-EP318937HWQ
CSB-BP318937HWQ
CSB-MP318937HWQ
CSB-EP318937HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia salina Elongation factor 1-beta CSB-YP318938AOE
CSB-EP318938AOE
CSB-BP318938AOE
CSB-MP318938AOE
CSB-EP318938AOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Fimbrial subunit type 3 (mrkA) CSB-BP318939KBG
CSB-MP318939KBG
CSB-EP318939KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Tryptophan synthase alpha chain (trpA) CSB-YP318941DQO
CSB-EP318941DQO
CSB-BP318941DQO
CSB-MP318941DQO
CSB-EP318941DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Glucose-1-phosphate adenylyltransferase large subunit, chloroplastic/amyloplastic (AGP-L) CSB-YP318942TQN
CSB-EP318942TQN
CSB-BP318942TQN
CSB-MP318942TQN
CSB-EP318942TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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