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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M3 ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP317445SMW
CSB-EP317445SMW
CSB-BP317445SMW
CSB-MP317445SMW
CSB-EP317445SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Cytidylate kinase (cmk) CSB-YP317446SMW
CSB-EP317446SMW
CSB-BP317446SMW
CSB-MP317446SMW
CSB-EP317446SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dephospho-CoA kinase (coaE) CSB-YP317447SMV
CSB-EP317447SMV
CSB-BP317447SMV
CSB-MP317447SMV
CSB-EP317447SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP317448SMW
CSB-EP317448SMW
CSB-BP317448SMW
CSB-MP317448SMW
CSB-EP317448SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 D-alanine--D-alanine ligase (ddl) CSB-YP317449SMW
CSB-EP317449SMW
CSB-BP317449SMW
CSB-MP317449SMW
CSB-EP317449SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DegV domain-containing protein SpyM3_1478 (SpyM3_1478) CSB-YP317450SMV
CSB-EP317450SMV
CSB-BP317450SMV
CSB-MP317450SMV
CSB-EP317450SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 DegV domain-containing protein SPs1668 (SPs1668) CSB-YP317451SMW
CSB-EP317451SMW
CSB-BP317451SMW
CSB-MP317451SMW
CSB-EP317451SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Deoxyribose-phosphate aldolase (deoC) CSB-YP317452SMW
CSB-EP317452SMW
CSB-BP317452SMW
CSB-MP317452SMW
CSB-EP317452SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal replication initiator protein DnaA (dnaA) CSB-YP317453SMV
CSB-EP317453SMV
CSB-BP317453SMV
CSB-MP317453SMV
CSB-EP317453SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 DNA ligase (ligA), partial CSB-YP317454SMW
CSB-EP317454SMW
CSB-BP317454SMW
CSB-MP317454SMW
CSB-EP317454SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase IV (dinB) CSB-YP317455SMV
CSB-EP317455SMV
CSB-BP317455SMV
CSB-MP317455SMV
CSB-EP317455SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 GTPase Der (der) CSB-YP317456SMW
CSB-EP317456SMW
CSB-BP317456SMW
CSB-MP317456SMW
CSB-EP317456SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Ferredoxin--NADP reductase (SPs1279) CSB-YP317457SMW
CSB-EP317457SMW
CSB-BP317457SMW
CSB-MP317457SMW
CSB-EP317457SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroneopterin aldolase (folB) CSB-YP317458SMV
CSB-EP317458SMV
CSB-BP317458SMV
CSB-MP317458SMV
CSB-EP317458SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP317459SMV
CSB-EP317459SMV
CSB-BP317459SMV
CSB-MP317459SMV
CSB-EP317459SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP317460SMV
CSB-EP317460SMV
CSB-BP317460SMV
CSB-MP317460SMV
CSB-EP317460SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP317461SMW
CSB-EP317461SMW
CSB-BP317461SMW
CSB-MP317461SMW
CSB-EP317461SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP317462SMV
CSB-EP317462SMV
CSB-BP317462SMV
CSB-MP317462SMV
CSB-EP317462SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 GTPase obg (obg) CSB-YP317463SMW
CSB-EP317463SMW
CSB-BP317463SMW
CSB-MP317463SMW
CSB-EP317463SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Hyaluronan synthase (hasA), partial CSB-YP317464SMW1
CSB-EP317464SMW1
CSB-BP317464SMW1
CSB-MP317464SMW1
CSB-EP317464SMW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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