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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bothrops insularis Bothroinsularin subunit beta CSB-YP317506BIX
CSB-EP317506BIX
CSB-BP317506BIX
CSB-MP317506BIX
CSB-EP317506BIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Reticulon-like protein B23 (RTNLB23), partial CSB-YP317507DOA1
CSB-EP317507DOA1
CSB-BP317507DOA1
CSB-MP317507DOA1
CSB-EP317507DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhG2 (mnhG2), partial CSB-YP317508SKV1
CSB-EP317508SKV1
CSB-BP317508SKV1
CSB-MP317508SKV1
CSB-EP317508SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Endo-type membrane-bound lytic murein transglycosylase A (emtA) CSB-YP317509ENV
CSB-EP317509ENV
CSB-BP317509ENV
CSB-MP317509ENV
CSB-EP317509ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Probable DNA-directed RNA polymerase subunit 1 homolog (Pret-111), partial CSB-YP317510AYG
CSB-EP317510AYG
CSB-BP317510AYG
CSB-MP317510AYG
CSB-EP317510AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus mRNA-decapping protein g5R (Pret-114) CSB-YP317511AYG
CSB-EP317511AYG
CSB-BP317511AYG
CSB-MP317511AYG
CSB-EP317511AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative DNA-directed RNA polymerase subunit 5 homolog (Ken-120) CSB-YP317512AEB
CSB-EP317512AEB
CSB-BP317512AEB
CSB-MP317512AEB
CSB-EP317512AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus DNA topoisomerase 2 (Pret-124), partial CSB-YP317513AYG
CSB-EP317513AYG
CSB-BP317513AYG
CSB-MP317513AYG
CSB-EP317513AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Probable AP endonuclease (Ken-146) CSB-YP317514AEB
CSB-EP317514AEB
CSB-BP317514AEB
CSB-MP317514AEB
CSB-EP317514AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative poly (A) polymerase catalytic subunit CSB-YP317515AEI
CSB-EP317515AEI
CSB-BP317515AEI
CSB-MP317515AEI
CSB-EP317515AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Trans-prenyltransferase (Pret-086), partial CSB-YP317516AYG
CSB-EP317516AYG
CSB-BP317516AYG
CSB-MP317516AYG
CSB-EP317516AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Viral histone-like protein A104R (War-045) CSB-YP317517AEI
CSB-EP317517AEI
CSB-BP317517AEI
CSB-MP317517AEI
CSB-EP317517AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 100-3L (Ken-163) CSB-YP317518AEB
CSB-EP317518AEB
CSB-BP317518AEB
CSB-MP317518AEB
CSB-EP317518AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-2L (Ken-008) CSB-YP317519AEB
CSB-EP317519AEB
CSB-BP317519AEB
CSB-MP317519AEB
CSB-EP317519AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-6L (War-013) CSB-YP317520AEI
CSB-EP317520AEI
CSB-BP317520AEI
CSB-MP317520AEI
CSB-EP317520AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-7L (Mal-013) CSB-YP317521AYE
CSB-EP317521AYE
CSB-BP317521AYE
CSB-MP317521AYE
CSB-EP317521AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-9L (Ken-019), partial CSB-YP317522AEB1
CSB-EP317522AEB1
CSB-BP317522AEB1
CSB-MP317522AEB1
CSB-EP317522AEB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-12L (Ken-021), partial CSB-YP317523AEB1
CSB-EP317523AEB1
CSB-BP317523AEB1
CSB-MP317523AEB1
CSB-EP317523AEB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 300-1L (Ken-027), partial CSB-YP317524AEB
CSB-EP317524AEB
CSB-BP317524AEB
CSB-MP317524AEB
CSB-EP317524AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 300-2R (Pret-028) CSB-YP317525AYG
CSB-EP317525AYG
CSB-BP317525AYG
CSB-MP317525AYG
CSB-EP317525AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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