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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella typhimurium E3 ubiquitin-protein ligase sspH2 (sspH2), partial CSB-YP317606SXB
CSB-EP317606SXB
CSB-BP317606SXB
CSB-MP317606SXB
CSB-EP317606SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus capitaneus Alpha-conotoxin-like Cp20.1 CSB-YP317607DVV
CSB-EP317607DVV
CSB-BP317607DVV
CSB-MP317607DVV
CSB-EP317607DVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus rattus Alpha-conotoxin-like Rt20.1 CSB-YP317608CPE
CSB-EP317608CPE
CSB-BP317608CPE
CSB-MP317608CPE
CSB-EP317608CPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucuronide carrier protein (uidB), partial CSB-YP317609ENV
CSB-EP317609ENV
CSB-BP317609ENV
CSB-MP317609ENV
CSB-EP317609ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insH for insertion sequence element IS5B (insH2) CSB-YP317610ENV
CSB-EP317610ENV
CSB-BP317610ENV
CSB-MP317610ENV
CSB-EP317610ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insH for insertion sequence element IS5-12 (JW1882) CSB-YP317611ENV
CSB-EP317611ENV
CSB-BP317611ENV
CSB-MP317611ENV
CSB-EP317611ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C3orf79 (C3orf79) CSB-YP317612HU
CSB-EP317612HU
CSB-BP317612HU
CSB-MP317612HU
CSB-EP317612HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Oncomodulin-1 (OCM) CSB-YP317613HU
CSB-EP317613HU
CSB-BP317613HU
CSB-MP317613HU
CSB-EP317613HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus stercusmuscarum Kappa-conotoxin-like SmIVA CSB-YP317614CPG
CSB-EP317614CPG
CSB-BP317614CPG
CSB-MP317614CPG
CSB-EP317614CPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxosceles intermedia Phospholipase D LiSicTox-alphaIA1a CSB-YP317615LRA
CSB-BP317615LRA
CSB-MP317615LRA
CSB-EP317615LRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Seripauperin-1 (PAU1) CSB-YP317616SVG
CSB-EP317616SVG
CSB-BP317616SVG
CSB-MP317616SVG
CSB-EP317616SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Seripauperin-6 (PAU6) CSB-YP317617SVG
CSB-EP317617SVG
CSB-BP317617SVG
CSB-MP317617SVG
CSB-EP317617SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoponera australis Dinoponeratoxin Da-1837 CSB-YP317618DEB
CSB-EP317618DEB
CSB-BP317618DEB
CSB-MP317618DEB
CSB-EP317618DEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Insertion element IS1 1 protein insA (insA1) CSB-YP317619ENV
CSB-EP317619ENV
CSB-BP317619ENV
CSB-MP317619ENV
CSB-EP317619ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Insertion element IS1 6 protein insA (insA6) CSB-YP317620ENV
CSB-EP317620ENV
CSB-BP317620ENV
CSB-MP317620ENV
CSB-EP317620ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UPF0507 protein YML003W (YML003W) CSB-YP317621SVG
CSB-EP317621SVG
CSB-BP317621SVG
CSB-MP317621SVG
CSB-EP317621SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae L-serine dehydratase (SDL1) CSB-YP317622SAC
CSB-EP317622SAC
CSB-BP317622SAC
CSB-MP317622SAC
CSB-EP317622SAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus discrepans Bactridin-1 CSB-YP317623TOM
CSB-EP317623TOM
CSB-BP317623TOM
CSB-MP317623TOM
CSB-EP317623TOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insC for insertion element IS2 on F plasmid (insC7) CSB-YP317624ENV
CSB-EP317624ENV
CSB-BP317624ENV
CSB-MP317624ENV
CSB-EP317624ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insC for insertion element IS2-11 (JW5934) CSB-YP317625ENV
CSB-EP317625ENV
CSB-BP317625ENV
CSB-MP317625ENV
CSB-EP317625ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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