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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D AdoMet-dependent rRNA methyltransferase SPB1 (SPB1), partial CSB-YP317809CTK
CSB-EP317809CTK
CSB-BP317809CTK
CSB-MP317809CTK
CSB-EP317809CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C1348.15 (SPBC1348.15) CSB-YP317810SXV
CSB-EP317810SXV
CSB-BP317810SXV
CSB-MP317810SXV
CSB-EP317810SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fragaria vesca (3S,6E)-nerolidol synthase 1, chloroplastic, partial CSB-YP317811FFA
CSB-EP317811FFA
CSB-BP317811FFA
CSB-MP317811FFA
CSB-EP317811FFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Testis-specific Y-encoded protein 4 (TSPY4) CSB-YP317812HU
CSB-EP317812HU
CSB-BP317812HU
CSB-MP317812HU
CSB-EP317812HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Chaperone protein DnaK (dnaK), partial CSB-YP317815MFC
CSB-EP317815MFC
CSB-BP317815MFC
CSB-MP317815MFC
CSB-EP317815MFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C18orf42 (C18orf42) CSB-YP317816HU
CSB-EP317816HU
CSB-BP317816HU
CSB-MP317816HU
CSB-EP317816HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB23 (vapB23) CSB-YP317817MVZ
CSB-EP317817MVZ
CSB-BP317817MVZ
CSB-MP317817MVZ
CSB-EP317817MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocaldococcus jannaschii Putative antitoxin VapB1 (vapB1) CSB-YP317818MRU
CSB-EP317818MRU
CSB-BP317818MRU
CSB-MP317818MRU
CSB-EP317818MRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nitrogen fixation nifHD region glnB-like protein 1 (glnBI) CSB-YP317819MSB
CSB-EP317819MSB
CSB-BP317819MSB
CSB-MP317819MSB
CSB-EP317819MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nitrogenase molybdenum-iron protein alpha chain (nifD) CSB-YP317820MEY
CSB-EP317820MEY
CSB-BP317820MEY
CSB-MP317820MEY
CSB-EP317820MEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nitrogenase iron protein (nifH) CSB-YP317821MEY
CSB-EP317821MEY
CSB-BP317821MEY
CSB-MP317821MEY
CSB-EP317821MEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA repair and recombination protein radB (radB) CSB-YP317822MEY
CSB-EP317822MEY
CSB-BP317822MEY
CSB-MP317822MEY
CSB-EP317822MEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Lysine--tRNA ligase (lysS), partial CSB-YP317823MEY
CSB-EP317823MEY
CSB-BP317823MEY
CSB-MP317823MEY
CSB-EP317823MEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Secreted protein BARF1 (BARF1) CSB-YP317825EFC
CSB-BP317825EFC
CSB-MP317825EFC
CSB-EP317825EFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative inactive cadmium/zinc-transporting ATPase HMA3 (HMA3), partial CSB-YP317826DOA1
CSB-EP317826DOA1
CSB-BP317826DOA1
CSB-MP317826DOA1
CSB-EP317826DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multidrug resistance protein EbrB (ebrB), partial CSB-YP317827BQH1
CSB-EP317827BQH1
CSB-BP317827BQH1
CSB-MP317827BQH1
CSB-EP317827BQH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter hansenii Cellulose-complementing protein (ccpAX) CSB-YP317828GCQ
CSB-EP317828GCQ
CSB-BP317828GCQ
CSB-MP317828GCQ
CSB-EP317828GCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Lysine--tRNA ligase (lysS), partial CSB-YP317829DRA
CSB-EP317829DRA
CSB-BP317829DRA
CSB-MP317829DRA
CSB-EP317829DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein PLANT CADMIUM RESISTANCE 3 (PCR3), partial CSB-YP317830DOA1
CSB-EP317830DOA1
CSB-BP317830DOA1
CSB-MP317830DOA1
CSB-EP317830DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum DNA-directed RNA polymerase subunit H (rpoH) CSB-YP317832HTM
CSB-EP317832HTM
CSB-BP317832HTM
CSB-MP317832HTM
CSB-EP317832HTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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