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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Molybdopterin molybdenumtransferase (moeA) CSB-YP318220ENV
CSB-EP318220ENV
CSB-BP318220ENV
CSB-MP318220ENV
CSB-EP318220ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Tryptophan synthase beta chain (trpB) CSB-YP318221DQO
CSB-EP318221DQO
CSB-BP318221DQO
CSB-MP318221DQO
CSB-EP318221DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Genome polyprotein CSB-YP318222MDX
CSB-EP318222MDX
CSB-BP318222MDX
CSB-MP318222MDX
CSB-EP318222MDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem II reaction center protein T (psbT), partial CSB-YP318223TNI
CSB-EP318223TNI
CSB-BP318223TNI
CSB-MP318223TNI
CSB-EP318223TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate phosphoribosyltransferase (trpD) CSB-YP318224SYN
CSB-EP318224SYN
CSB-BP318224SYN
CSB-MP318224SYN
CSB-EP318224SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorophyll a-b binding protein of LHCII type 1, partial CSB-YP318225EHU1
CSB-EP318225EHU1
CSB-BP318225EHU1
CSB-MP318225EHU1
CSB-EP318225EHU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Silene pratensis Chlorophyll a-b binding protein, chloroplastic, partial CSB-YP318226SGM1
CSB-EP318226SGM1
CSB-BP318226SGM1
CSB-MP318226SGM1
CSB-EP318226SGM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azurin iso-2 CSB-YP318227MFO
CSB-EP318227MFO
CSB-BP318227MFO
CSB-MP318227MFO
CSB-EP318227MFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 30S ribosomal protein S7, chloroplastic (rps7-A) CSB-YP318228ZAX
CSB-EP318228ZAX
CSB-BP318228ZAX
CSB-MP318228ZAX
CSB-EP318228ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Photosystem I reaction center subunit IV, chloroplastic (PSAE) CSB-YP318229DSJ
CSB-EP318229DSJ
CSB-BP318229DSJ
CSB-MP318229DSJ
CSB-EP318229DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Photosystem I reaction center subunit III, chloroplastic (PSAF) CSB-YP318230FKI
CSB-EP318230FKI
CSB-BP318230FKI
CSB-MP318230FKI
CSB-EP318230FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP318231HWQ1
CSB-EP318231HWQ1
CSB-BP318231HWQ1
CSB-MP318231HWQ1
CSB-EP318231HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Copper resistance protein C (copC) CSB-YP318232PWG
CSB-BP318232PWG
CSB-MP318232PWG
CSB-EP318232PWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Reovirus type 2 Outer capsid protein mu-1 (M2), partial CSB-YP318233RCG
CSB-EP318233RCG
CSB-BP318233RCG
CSB-MP318233RCG
CSB-EP318233RCG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein CTLA-2-beta (Ctla2b) CSB-YP318234MO
CSB-EP318234MO
CSB-BP318234MO
CSB-MP318234MO
CSB-EP318234MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. ATP synthase gamma chain (atpG) CSB-YP318235NHR
CSB-EP318235NHR
CSB-BP318235NHR
CSB-MP318235NHR
CSB-EP318235NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vigna mungo Vignain CSB-YP318236VCV
CSB-EP318236VCV
CSB-BP318236VCV
CSB-MP318236VCV
CSB-EP318236VCV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mamestra brassicae nuclear polyhedrosis virus Polyhedrin (PH) CSB-YP318237MBU
CSB-EP318237MBU
CSB-BP318237MBU
CSB-MP318237MBU
CSB-EP318237MBU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Actin, muscle CSB-YP318238FOT
CSB-EP318238FOT
CSB-BP318238FOT
CSB-MP318238FOT
CSB-EP318238FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces microflavus Isopenicillin N synthase (pcbC) CSB-YP318239SOW
CSB-EP318239SOW
CSB-BP318239SOW
CSB-MP318239SOW
CSB-EP318239SOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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