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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Avian infectious bronchitis virus Membrane protein (M), partial CSB-YP318841ARL1
CSB-EP318841ARL1
CSB-BP318841ARL1
CSB-MP318841ARL1
CSB-EP318841ARL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Putative transposase element L1Md-A101/L1Md-A102/L1Md-A2 CSB-YP318842MO
CSB-BP318842MO
CSB-MP318842MO
CSB-EP318842MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Envelope glycoprotein gp160 (env), partial CSB-YP318843SHA
CSB-EP318843SHA
CSB-BP318843SHA
CSB-MP318843SHA
CSB-EP318843SHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian erythroblastosis virus Tyrosine-protein kinase transforming protein erbB (V-ERBB), partial CSB-YP318844ARI
CSB-EP318844ARI
CSB-BP318844ARI
CSB-MP318844ARI
CSB-EP318844ARI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse mammary tumor virus Gag polyprotein (gag) CSB-YP318845MIU
CSB-EP318845MIU
CSB-BP318845MIU
CSB-MP318845MIU
CSB-EP318845MIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Woodchuck hepatitis B virus Protein X (X) CSB-YP318846WAX
CSB-EP318846WAX
CSB-BP318846WAX
CSB-MP318846WAX
CSB-EP318846WAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable protein E3 (E3) CSB-YP318847BKK
CSB-EP318847BKK
CSB-BP318847BKK
CSB-MP318847BKK
CSB-EP318847BKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 3 Early E3B 14.5 kDa protein, partial CSB-YP318848HIG1
CSB-EP318848HIG1
CSB-BP318848HIG1
CSB-MP318848HIG1
CSB-EP318848HIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 3 Early E3 16 kDa glycoprotein CSB-YP318849HIG
CSB-EP318849HIG
CSB-BP318849HIG
CSB-MP318849HIG
CSB-EP318849HIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 3 Hexon-associated protein (PVIII) CSB-YP318850HIG
CSB-EP318850HIG
CSB-BP318850HIG
CSB-MP318850HIG
CSB-EP318850HIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European elk papillomavirus Replication protein E1 (E1), partial CSB-YP318851EIN
CSB-EP318851EIN
CSB-BP318851EIN
CSB-MP318851EIN
CSB-EP318851EIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus 4 Protein ORF5 (ORF5) CSB-YP318852SJY
CSB-EP318852SJY
CSB-BP318852SJY
CSB-MP318852SJY
CSB-EP318852SJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus 4 Uncharacterized protein ORF7 (ORF7), partial CSB-YP318853SJY
CSB-EP318853SJY
CSB-BP318853SJY
CSB-MP318853SJY
CSB-EP318853SJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Respiratory nitrate reductase 1 gamma chain (narI), partial CSB-YP318854ENV1
CSB-EP318854ENV1
CSB-BP318854ENV1
CSB-MP318854ENV1
CSB-EP318854ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant T-cell receptor beta chain T17T-22 (V-TCR) CSB-YP318855FAN
CSB-EP318855FAN
CSB-BP318855FAN
CSB-MP318855FAN
CSB-EP318855FAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyridium cruentum B-phycoerythrin alpha chain (cpeA) CSB-YP318856PQN
CSB-EP318856PQN
CSB-BP318856PQN
CSB-MP318856PQN
CSB-EP318856PQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mastigocladus laminosus Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod (cpcC) CSB-YP318857MQJ
CSB-EP318857MQJ
CSB-BP318857MQJ
CSB-MP318857MQJ
CSB-EP318857MQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Phycobilisome 37.5 kDa linker polypeptide, phycocyanin-associated, rod (cpcH2) CSB-YP318858FCY
CSB-EP318858FCY
CSB-BP318858FCY
CSB-MP318858FCY
CSB-EP318858FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyberlindnera jadinii Glucose-6-phosphate 1-dehydrogenase CSB-YP318859EPX
CSB-EP318859EPX
CSB-BP318859EPX
CSB-MP318859EPX
CSB-EP318859EPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana otophora Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP318860NDL
CSB-EP318860NDL
CSB-BP318860NDL
CSB-MP318860NDL
CSB-EP318860NDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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