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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Replication initiation protein (repA1) CSB-YP318448ENL
CSB-EP318448ENL
CSB-BP318448ENL
CSB-MP318448ENL
CSB-EP318448ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein kleA (kleA) CSB-YP318449ENL
CSB-EP318449ENL
CSB-BP318449ENL
CSB-MP318449ENL
CSB-EP318449ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein kleD (kleD) CSB-YP318450ENL
CSB-EP318450ENL
CSB-BP318450ENL
CSB-MP318450ENL
CSB-EP318450ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Relaxosome protein TraM (traM) CSB-YP318451ENL
CSB-EP318451ENL
CSB-BP318451ENL
CSB-MP318451ENL
CSB-EP318451ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DegV domain-containing 15.5 kDa protein CSB-YP318452FKZ
CSB-EP318452FKZ
CSB-BP318452FKZ
CSB-MP318452FKZ
CSB-EP318452FKZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Arginine deiminase (arcA) CSB-YP318453EZX
CSB-EP318453EZX
CSB-BP318453EZX
CSB-MP318453EZX
CSB-EP318453EZX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Extensin (HRGPNT3), partial CSB-YP318454NHE
CSB-EP318454NHE
CSB-BP318454NHE
CSB-MP318454NHE
CSB-EP318454NHE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposon Tn7 transposition protein tnsD (tnsD) CSB-YP318455ENL
CSB-EP318455ENL
CSB-BP318455ENL
CSB-MP318455ENL
CSB-EP318455ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein hairy (h) CSB-YP318457DLU
CSB-EP318457DLU
CSB-BP318457DLU
CSB-MP318457DLU
CSB-EP318457DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota 14 kDa proline-rich protein DC2.15, partial CSB-YP318458DIR1
CSB-EP318458DIR1
CSB-BP318458DIR1
CSB-MP318458DIR1
CSB-EP318458DIR1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore coat protein E (cotE) CSB-YP318459BRJ
CSB-EP318459BRJ
CSB-BP318459BRJ
CSB-MP318459BRJ
CSB-EP318459BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L19e (rpl19e) CSB-YP318460MEZ
CSB-EP318460MEZ
CSB-BP318460MEZ
CSB-MP318460MEZ
CSB-EP318460MEZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14P (rpl14p) CSB-YP318461MEZ
CSB-EP318461MEZ
CSB-BP318461MEZ
CSB-MP318461MEZ
CSB-EP318461MEZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S5P (rps5p) CSB-YP318462MEZ
CSB-EP318462MEZ
CSB-BP318462MEZ
CSB-MP318462MEZ
CSB-EP318462MEZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S8P (rps8p) CSB-YP318463MEZ
CSB-EP318463MEZ
CSB-BP318463MEZ
CSB-MP318463MEZ
CSB-EP318463MEZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S14P type Z (rps14p) CSB-YP318464MEZ
CSB-EP318464MEZ
CSB-BP318464MEZ
CSB-MP318464MEZ
CSB-EP318464MEZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Procyclic form-specific polypeptide (PROA) CSB-YP318465TQV
CSB-EP318465TQV
CSB-BP318465TQV
CSB-MP318465TQV
CSB-EP318465TQV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcriptional activator HAP4 (HAP4), partial CSB-YP318466SVG
CSB-EP318466SVG
CSB-BP318466SVG
CSB-MP318466SVG
CSB-EP318466SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubredoxin CSB-YP318467BOF
CSB-EP318467BOF
CSB-BP318467BOF
CSB-MP318467BOF
CSB-EP318467BOF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-cell leukemia virus 1 Gag polyprotein (gag) CSB-YP318468HQK
CSB-EP318468HQK
CSB-BP318468HQK
CSB-MP318468HQK
CSB-EP318468HQK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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