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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila grimshawi Chorion protein S15 (Cp15) CSB-YP318363DLL
CSB-EP318363DLL
CSB-BP318363DLL
CSB-MP318363DLL
CSB-EP318363DLL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Chorion protein S19 (Cp19) CSB-YP318364DLL
CSB-EP318364DLL
CSB-BP318364DLL
CSB-MP318364DLL
CSB-EP318364DLL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O6:K15:H31 S-fimbrial protein subunit SfaH (sfaH) CSB-YP318365EGY
CSB-EP318365EGY
CSB-BP318365EGY
CSB-MP318365EGY
CSB-EP318365EGY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcriptional activator HAP3 (HAP3) CSB-YP318366SVG
CSB-EP318366SVG
CSB-BP318366SVG
CSB-MP318366SVG
CSB-EP318366SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Bifunctional enzyme NodQ (nodQ), partial CSB-YP318368RKU
CSB-EP318368RKU
CSB-BP318368RKU
CSB-MP318368RKU
CSB-EP318368RKU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Anther-specific protein LAT52 (LAT52) CSB-YP318369SZY
CSB-EP318369SZY
CSB-BP318369SZY
CSB-MP318369SZY
CSB-EP318369SZY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant T-DNA border endonuclease virD2 (virD2) CSB-YP318370AEW
CSB-EP318370AEW
CSB-BP318370AEW
CSB-MP318370AEW
CSB-EP318370AEW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein virD4 (virD4), partial CSB-YP318371AEW1
CSB-EP318371AEW1
CSB-BP318371AEW1
CSB-MP318371AEW1
CSB-EP318371AEW1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster DNA-binding protein K10 (fs (1)K10) CSB-YP318372DLU
CSB-EP318372DLU
CSB-BP318372DLU
CSB-MP318372DLU
CSB-EP318372DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colicin-E9 immunity protein (imm) CSB-YP318373ENL
CSB-BP318373ENL
CSB-MP318373ENL
CSB-EP318373ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Oligo (A)/oligo (T)-binding protein CSB-YP318374SVG
CSB-EP318374SVG
CSB-BP318374SVG
CSB-MP318374SVG
CSB-EP318374SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Potassium channel toxin alpha-KTx 1.1 CSB-YP318375LDS
CSB-EP318375LDS
CSB-BP318375LDS
CSB-MP318375LDS
CSB-EP318375LDS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudonaja textilis Pseudonajatoxin b CSB-YP318376FGS
CSB-EP318376FGS
CSB-BP318376FGS
CSB-MP318376FGS
CSB-EP318376FGS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Glucan 1,4-alpha-maltotetraohydrolase (amyP), partial CSB-YP318377FGJ
CSB-EP318377FGJ
CSB-BP318377FGJ
CSB-MP318377FGJ
CSB-EP318377FGJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fusarium sporotrichioides Trichodiene synthase (TRI5) CSB-YP318378FFQ
CSB-EP318378FFQ
CSB-BP318378FFQ
CSB-MP318378FFQ
CSB-EP318378FFQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sucrose-6-phosphate hydrolase (scrB) CSB-YP318379FMR
CSB-EP318379FMR
CSB-BP318379FMR
CSB-MP318379FMR
CSB-EP318379FMR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plum pox potyvirus Genome polyprotein CSB-YP318381PNM
CSB-EP318381PNM
CSB-BP318381PNM
CSB-MP318381PNM
CSB-EP318381PNM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Chorion protein ERB.1 CSB-YP318382BTT
CSB-EP318382BTT
CSB-BP318382BTT
CSB-MP318382BTT
CSB-EP318382BTT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Photosystem II reaction center protein J (psbJ), partial CSB-YP318383EWE
CSB-EP318383EWE
CSB-BP318383EWE
CSB-MP318383EWE
CSB-EP318383EWE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage PRD1 Lysozyme (XV) CSB-YP318384EDO
CSB-EP318384EDO
CSB-BP318384EDO
CSB-MP318384EDO
CSB-EP318384EDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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