Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vesicular stomatitis Indiana virus Nucleoprotein (N) CSB-YP319266VBG
CSB-MP319266VBG
CSB-EP319266VBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial CSB-YP319267ARN
CSB-EP319267ARN
CSB-BP319267ARN
CSB-MP319267ARN
CSB-EP319267ARN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S19 (rpsS) CSB-YP319268YAH
CSB-EP319268YAH
CSB-BP319268YAH
CSB-MP319268YAH
CSB-EP319268YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Tat (tat) CSB-YP319269SHA
CSB-EP319269SHA
CSB-BP319269SHA
CSB-MP319269SHA
CSB-EP319269SHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Envelope glycoprotein (env), partial CSB-YP319270RAM
CSB-EP319270RAM
CSB-BP319270RAM
CSB-MP319270RAM
CSB-EP319270RAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Periplasmic pectate lyase (pelY), partial CSB-YP319271YAH
CSB-EP319271YAH
CSB-BP319271YAH
CSB-MP319271YAH
CSB-EP319271YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela vison Pro-opiomelanocortin (POMC) CSB-YP319272MVE
CSB-EP319272MVE
CSB-BP319272MVE
CSB-MP319272MVE
CSB-EP319272MVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein yiaB (yiaB), partial CSB-YP319273ENV1
CSB-EP319273ENV1
CSB-BP319273ENV1
CSB-MP319273ENV1
CSB-EP319273ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein b3808 (b3808, JW3780) CSB-YP319274ENV
CSB-EP319274ENV
CSB-BP319274ENV
CSB-MP319274ENV
CSB-EP319274ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-lysine 6-monooxygenase (iucD) CSB-YP319275ENL
CSB-EP319275ENL
CSB-BP319275ENL
CSB-MP319275ENL
CSB-EP319275ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein E8 CSB-YP319276BKK
CSB-EP319276BKK
CSB-BP319276BKK
CSB-MP319276BKK
CSB-EP319276BKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine infectious anemia virus Protein Rev (rev) CSB-YP319277EFS
CSB-EP319277EFS
CSB-BP319277EFS
CSB-MP319277EFS
CSB-EP319277EFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Adhesin P1 (mgpA), partial CSB-YP319278MLW
CSB-EP319278MLW
CSB-BP319278MLW
CSB-MP319278MLW
CSB-EP319278MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 3 Early E3 15.3 kDa protein CSB-YP319279HIG
CSB-EP319279HIG
CSB-BP319279HIG
CSB-MP319279HIG
CSB-EP319279HIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 3 Early E3 20.5 kDa glycoprotein CSB-YP319280HIG
CSB-EP319280HIG
CSB-BP319280HIG
CSB-MP319280HIG
CSB-EP319280HIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European elk papillomavirus Probable regulatory protein E2 (E2) CSB-YP319281EIN
CSB-EP319281EIN
CSB-BP319281EIN
CSB-MP319281EIN
CSB-EP319281EIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European elk papillomavirus Protein E7 (E7) CSB-YP319282EIN
CSB-EP319282EIN
CSB-BP319282EIN
CSB-MP319282EIN
CSB-EP319282EIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus 4 Minor spike protein H (ORF4) CSB-YP319283SJY
CSB-EP319283SJY
CSB-BP319283SJY
CSB-MP319283SJY
CSB-EP319283SJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Raf homolog serine/threonine-protein kinase phl (phl), partial CSB-YP319284DLU
CSB-EP319284DLU
CSB-BP319284DLU
CSB-MP319284DLU
CSB-EP319284DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Respiratory nitrate reductase 1 beta chain (narH) CSB-YP319285ENV
CSB-EP319285ENV
CSB-BP319285ENV
CSB-MP319285ENV
CSB-EP319285ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>