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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cucumis sativus Trypsin inhibitor 2b CSB-YP319879CXP
CSB-EP319879CXP
CSB-BP319879CXP
CSB-MP319879CXP
CSB-EP319879CXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytolacca americana Antiviral protein I (PAP1) CSB-YP319880PJI
CSB-EP319880PJI
CSB-BP319880PJI
CSB-MP319880PJI
CSB-EP319880PJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 Lysis protein (17.5) CSB-YP319881EDY
CSB-EP319881EDY
CSB-BP319881EDY
CSB-MP319881EDY
CSB-EP319881EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 DNA maturase B (19), partial CSB-YP319882EDY
CSB-EP319882EDY
CSB-BP319882EDY
CSB-MP319882EDY
CSB-EP319882EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HLA class I histocompatibility antigen, A-69 alpha chain (HLA-A), partial CSB-YP319883HU
CSB-EP319883HU
CSB-BP319883HU
CSB-MP319883HU
CSB-EP319883HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Peptidoglycan-associated lipoprotein (pal) CSB-YP319884HTA
CSB-BP319884HTA
CSB-MP319884HTA
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Accessory gland-specific peptide 26Aa (Acp26Aa) CSB-YP319885DLU
CSB-EP319885DLU
CSB-BP319885DLU
CSB-MP319885DLU
CSB-EP319885DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Prephenate dehydratase (pheA) CSB-YP319886DWY
CSB-EP319886DWY
CSB-BP319886DWY
CSB-MP319886DWY
CSB-EP319886DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba Photosystem II reaction center protein K (psbK) CSB-YP319887SZO
CSB-EP319887SZO
CSB-BP319887SZO
CSB-MP319887SZO
CSB-EP319887SZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bluetongue virus 10 Non-structural protein NS2 (S8) CSB-YP319888BHP
CSB-EP319888BHP
CSB-BP319888BHP
CSB-MP319888BHP
CSB-EP319888BHP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 69 kDa protein, partial CSB-YP319889TJO
CSB-EP319889TJO
CSB-BP319889TJO
CSB-MP319889TJO
CSB-EP319889TJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP319890SXB
CSB-EP319890SXB
CSB-BP319890SXB
CSB-MP319890SXB
CSB-EP319890SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein unzipped (uzip), partial CSB-YP319891DLU
CSB-EP319891DLU
CSB-BP319891DLU
CSB-MP319891DLU
CSB-EP319891DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Glutenin, low molecular weight subunit 1D1 CSB-YP319892TQN
CSB-BP319892TQN
CSB-MP319892TQN
CSB-EP319892TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein nifQ (nifQ) CSB-YP319893KBG
CSB-EP319893KBG
CSB-BP319893KBG
CSB-MP319893KBG
CSB-EP319893KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication initiation protein (repA) CSB-YP319894ENL
CSB-EP319894ENL
CSB-BP319894ENL
CSB-MP319894ENL
CSB-EP319894ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Retrovirus-related Pol polyprotein (POL) CSB-YP319895CH
CSB-EP319895CH
CSB-BP319895CH
CSB-MP319895CH
CSB-EP319895CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Retrovirus-related Gag polyprotein from transposon gypsy (gag) CSB-YP319896DLU
CSB-EP319896DLU
CSB-BP319896DLU
CSB-MP319896DLU
CSB-EP319896DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein translocase subunit SecA (secA), partial CSB-YP319897ENV
CSB-EP319897ENV
CSB-BP319897ENV
CSB-MP319897ENV
CSB-EP319897ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura elegantissima Antho-RFamide neuropeptides type 1 CSB-YP319898DNA
CSB-EP319898DNA
CSB-BP319898DNA
CSB-MP319898DNA
CSB-EP319898DNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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