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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Pisum sativum 6a-hydroxymaackiain methyltransferase 2 (HMM2) CSB-YP318678EWE
CSB-EP318678EWE
CSB-BP318678EWE
CSB-MP318678EWE
CSB-EP318678EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Putative regulatory protein PrgT (prgT) CSB-YP318679ELV
CSB-EP318679ELV
CSB-BP318679ELV
CSB-MP318679ELV
CSB-EP318679ELV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Barley B recombinant-like protein B (Os10g0115200, LOC_Os10g02584) CSB-YP318680OFG
CSB-EP318680OFG
CSB-BP318680OFG
CSB-MP318680OFG
CSB-EP318680OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g36820 (At5g36820) CSB-YP318681DOA
CSB-EP318681DOA
CSB-BP318681DOA
CSB-MP318681DOA
CSB-EP318681DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histidinol-phosphate aminotransferase 2, chloroplastic (HPA2) CSB-YP318682DOA
CSB-EP318682DOA
CSB-BP318682DOA
CSB-MP318682DOA
CSB-EP318682DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daboia russelli russelli L-amino-acid oxidase L2 CSB-YP318683DAA
CSB-EP318683DAA
CSB-BP318683DAA
CSB-MP318683DAA
CSB-EP318683DAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein p35 CSB-YP318684MLT
CSB-EP318684MLT
CSB-BP318684MLT
CSB-MP318684MLT
CSB-EP318684MLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterometrus petersii Potassium channel toxin kappa-KTx 2.7 CSB-YP318685HGR
CSB-EP318685HGR
CSB-BP318685HGR
CSB-MP318685HGR
CSB-EP318685HGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterometrus laoticus Potassium channel toxin kappa-KTx 5.1 CSB-YP318686HGQ
CSB-EP318686HGQ
CSB-BP318686HGQ
CSB-MP318686HGQ
CSB-EP318686HGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops leucurus Phospholipase A2, acidic CSB-YP318687BIY
CSB-EP318687BIY
CSB-BP318687BIY
CSB-MP318687BIY
CSB-EP318687BIY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum HNTX-0314934 CSB-YP318688HUK
CSB-EP318688HUK
CSB-BP318688HUK
CSB-MP318688HUK
CSB-EP318688HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum HNTX-0314968 CSB-YP318689HUK
CSB-EP318689HUK
CSB-BP318689HUK
CSB-MP318689HUK
CSB-EP318689HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti HWTX-XI-IS27 CSB-YP318690HUL
CSB-EP318690HUL
CSB-BP318690HUL
CSB-MP318690HUL
CSB-EP318690HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translocated intimin receptor Tir (tir), partial CSB-YP318691ENL
CSB-EP318691ENL
CSB-BP318691ENL
CSB-MP318691ENL
CSB-EP318691ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cellulosilyticus Putative Gly-rich membrane protein Bcell_0380 (Bcell_0380), partial CSB-YP318692BAL
CSB-EP318692BAL
CSB-BP318692BAL
CSB-MP318692BAL
CSB-EP318692BAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis HTH-type transcriptional regulator Zrp (zrp) CSB-YP318693ZBM
CSB-EP318693ZBM
CSB-BP318693ZBM
CSB-MP318693ZBM
CSB-EP318693ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Release factor glutamine methyltransferase (prmC) CSB-YP318694DWY
CSB-EP318694DWY
CSB-BP318694DWY
CSB-MP318694DWY
CSB-EP318694DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus californicus Conotoxin Cl9a CSB-YP318695DVU
CSB-EP318695DVU
CSB-BP318695DVU
CSB-MP318695DVU
CSB-EP318695DVU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus quercinus Conotoxin Qc6.1 CSB-YP318696CXE
CSB-EP318696CXE
CSB-BP318696CXE
CSB-MP318696CXE
CSB-EP318696CXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RNA-binding motif protein, Y chromosome, family 1 member C (RBMY1C) CSB-YP318697HU
CSB-EP318697HU
CSB-BP318697HU
CSB-MP318697HU
CSB-EP318697HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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