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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus phage phi105 Putative uncharacterized immunity region protein 6 CSB-YP319899BBA
CSB-EP319899BBA
CSB-BP319899BBA
CSB-MP319899BBA
CSB-EP319899BBA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi105 Uncharacterized immunity region protein 14 CSB-YP319900BBA
CSB-EP319900BBA
CSB-BP319900BBA
CSB-MP319900BBA
CSB-EP319900BBA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage PA-2 Lysozyme (15) CSB-YP319901EDJ
CSB-EP319901EDJ
CSB-BP319901EDJ
CSB-MP319901EDJ
CSB-EP319901EDJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA), partial CSB-YP319902IIV
CSB-EP319902IIV
CSB-BP319902IIV
CSB-MP319902IIV
CSB-EP319902IIV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laticauda colubrina Short neurotoxin C CSB-YP319903LCZ
CSB-EP319903LCZ
CSB-BP319903LCZ
CSB-MP319903LCZ
CSB-EP319903LCZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laticauda colubrina Short neurotoxin II CSB-YP319904LCZ
CSB-EP319904LCZ
CSB-BP319904LCZ
CSB-MP319904LCZ
CSB-EP319904LCZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus X TGB2 protein (ORF3), partial CSB-YP319905PRG
CSB-EP319905PRG
CSB-BP319905PRG
CSB-MP319905PRG
CSB-EP319905PRG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Endo-1,4-beta-xylanase Z (xynZ), partial CSB-YP319906DUT
CSB-EP319906DUT
CSB-BP319906DUT
CSB-MP319906DUT
CSB-EP319906DUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type I restriction enzyme EcoR124II R protein (hsdR), partial CSB-YP319907ENL
CSB-EP319907ENL
CSB-BP319907ENL
CSB-MP319907ENL
CSB-EP319907ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Anaphase-promoting complex subunit 3 (nuc2), partial CSB-YP319909SXV
CSB-EP319909SXV
CSB-BP319909SXV
CSB-MP319909SXV
CSB-EP319909SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein traE (traE) CSB-YP319910ENL
CSB-EP319910ENL
CSB-BP319910ENL
CSB-MP319910ENL
CSB-EP319910ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose 1-dehydrogenase A (gdhA) CSB-YP319911BQV
CSB-EP319911BQV
CSB-BP319911BQV
CSB-MP319911BQV
CSB-EP319911BQV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem II manganese-stabilizing polypeptide (psbO) CSB-YP319912SSQ
CSB-EP319912SSQ
CSB-BP319912SSQ
CSB-MP319912SSQ
CSB-EP319912SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba castellanii Myosin IC heavy chain (MIC), partial CSB-YP319913AAJ
CSB-EP319913AAJ
CSB-BP319913AAJ
CSB-MP319913AAJ
CSB-EP319913AAJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Uncharacterized 29 kDa protein in mitochondrial S-1 DNA CSB-YP319914ZAX
CSB-EP319914ZAX
CSB-BP319914ZAX
CSB-MP319914ZAX
CSB-EP319914ZAX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Heat shock protein SSA1 (SSA1), partial CSB-YP319915SVG
CSB-BP319915SVG
CSB-MP319915SVG
CSB-EP319915SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S acidic ribosomal protein P1-beta (RPP1B) CSB-YP319916SVG
CSB-EP319916SVG
CSB-BP319916SVG
CSB-MP319916SVG
CSB-EP319916SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plastocyanin (petE) CSB-YP319917MOA
CSB-EP319917MOA
CSB-BP319917MOA
CSB-MP319917MOA
CSB-EP319917MOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-binding protein BexA (bexA) CSB-YP319918HSZ
CSB-EP319918HSZ
CSB-BP319918HSZ
CSB-MP319918HSZ
CSB-EP319918HSZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Ribulose bisphosphate carboxylase small chain C, chloroplastic (RBCS-C) CSB-YP319919FIG
CSB-EP319919FIG
CSB-BP319919FIG
CSB-MP319919FIG
CSB-EP319919FIG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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