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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Kluyveromyces lactis Phosphoglycerate kinase (PGK) CSB-YP318598KBK
CSB-EP318598KBK
CSB-BP318598KBK
CSB-MP318598KBK
CSB-EP318598KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Peptidyl-prolyl cis-trans isomerase (CPR1) CSB-YP318599SVG
CSB-EP318599SVG
CSB-BP318599SVG
CSB-MP318599SVG
CSB-EP318599SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artichoke mottled crinkle virus Capsid protein (ORF2) CSB-YP318600AOX
CSB-EP318600AOX
CSB-BP318600AOX
CSB-MP318600AOX
CSB-EP318600AOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited (ARO3) CSB-YP318601SVG
CSB-EP318601SVG
CSB-BP318601SVG
CSB-MP318601SVG
CSB-EP318601SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP318602SXB
CSB-EP318602SXB
CSB-BP318602SXB
CSB-MP318602SXB
CSB-EP318602SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leghemoglobin 2 (GLB2) CSB-YP318603SYQ
CSB-EP318603SYQ
CSB-BP318603SYQ
CSB-MP318603SYQ
CSB-EP318603SYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Peptidyl-prolyl cis-trans isomerase A (PPIA) CSB-YP318604DXU
CSB-EP318604DXU
CSB-BP318604DXU
CSB-MP318604DXU
CSB-EP318604DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucor circinelloides f. lusitanicus Elongation factor 1-alpha (TEF-3) CSB-YP318605MIW
CSB-EP318605MIW
CSB-BP318605MIW
CSB-MP318605MIW
CSB-EP318605MIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Planomicrobium okeanokoites Type-2 restriction enzyme FokI (fokIR), partial CSB-YP318606PLE
CSB-EP318606PLE
CSB-BP318606PLE
CSB-MP318606PLE
CSB-EP318606PLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon C-phycocyanin-3 alpha chain (cpcA3) CSB-YP318607FCY
CSB-EP318607FCY
CSB-BP318607FCY
CSB-MP318607FCY
CSB-EP318607FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Phycobilisome 31.6 kDa linker polypeptide, phycocyanin-associated, rod (cpcI3) CSB-YP318608FCY
CSB-EP318608FCY
CSB-BP318608FCY
CSB-MP318608FCY
CSB-EP318608FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative peptidyl-prolyl cis-trans isomerase NifM (nifM) CSB-YP318609DPX
CSB-EP318609DPX
CSB-BP318609DPX
CSB-MP318609DPX
CSB-EP318609DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (HSD3B), partial CSB-YP318610BO1
CSB-EP318610BO1
CSB-BP318610BO1
CSB-MP318610BO1
CSB-EP318610BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Low molecular mass early light-inducible protein HV90, chloroplastic, partial CSB-YP318611HWQ1
CSB-EP318611HWQ1
CSB-BP318611HWQ1
CSB-MP318611HWQ1
CSB-EP318611HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP318612ENV
CSB-EP318612ENV
CSB-BP318612ENV
CSB-MP318612ENV
CSB-EP318612ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar aminopeptidase 1 (LAP4) CSB-YP318613SVG
CSB-EP318613SVG
CSB-BP318613SVG
CSB-MP318613SVG
CSB-EP318613SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein translocation protein SEC63 (SEC63), partial CSB-YP318614SVG1
CSB-EP318614SVG1
CSB-BP318614SVG1
CSB-MP318614SVG1
CSB-EP318614SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum 4-coumarate--CoA ligase 1 (4CL2), partial CSB-YP318615PGL
CSB-EP318615PGL
CSB-BP318615PGL
CSB-MP318615PGL
CSB-EP318615PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Signal recognition particle receptor FtsY (ftsY) CSB-YP318616NFZ
CSB-EP318616NFZ
CSB-BP318616NFZ
CSB-MP318616NFZ
CSB-EP318616NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Uncharacterized protein MTBMA_p00020 (MTBMA_p00020) CSB-YP318617MSQ
CSB-EP318617MSQ
CSB-BP318617MSQ
CSB-MP318617MSQ
CSB-EP318617MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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