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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Wound tumor virus Non-structural protein PNS7 (S6), partial CSB-YP319492WBC
CSB-EP319492WBC
CSB-BP319492WBC
CSB-MP319492WBC
CSB-EP319492WBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Enhancer of split m5 protein (HLHm5) CSB-YP319493DLU
CSB-EP319493DLU
CSB-BP319493DLU
CSB-MP319493DLU
CSB-EP319493DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP319494IIK
CSB-EP319494IIK
CSB-BP319494IIK
CSB-MP319494IIK
CSB-EP319494IIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Fibroblast growth factor 2 (Fgf2) CSB-YP319495RA
CSB-EP319495RA
CSB-BP319495RA
CSB-MP319495RA
CSB-EP319495RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Flagellin A (flaA) CSB-YP319496RKT
CSB-EP319496RKT
CSB-BP319496RKT
CSB-MP319496RKT
CSB-EP319496RKT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hexagonally packed intermediate-layer surface protein (hpi), partial CSB-YP319497DBB
CSB-EP319497DBB
CSB-BP319497DBB
CSB-MP319497DBB
CSB-EP319497DBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Luciola cruciata Luciferin 4-monooxygenase, partial CSB-YP319498LJK
CSB-EP319498LJK
CSB-BP319498LJK
CSB-MP319498LJK
CSB-EP319498LJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Kexin (KEX2), partial CSB-YP319499SVG
CSB-EP319499SVG
CSB-BP319499SVG
CSB-MP319499SVG
CSB-EP319499SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP319500IFW
CSB-EP319500IFW
CSB-BP319500IFW
CSB-MP319500IFW
CSB-EP319500IFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP319501IFU
CSB-EP319501IFU
CSB-BP319501IFU
CSB-MP319501IFU
CSB-EP319501IFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Leucine dehydrogenase (ldh) CSB-YP319502GFM
CSB-EP319502GFM
CSB-BP319502GFM
CSB-MP319502GFM
CSB-EP319502GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline herpesvirus 1 Protein UL24 homolog (UL24) CSB-YP319503FAJ
CSB-EP319503FAJ
CSB-BP319503FAJ
CSB-MP319503FAJ
CSB-EP319503FAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP319504IEP
CSB-EP319504IEP
CSB-BP319504IEP
CSB-MP319504IEP
CSB-EP319504IEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP319505IGV
CSB-EP319505IGV
CSB-BP319505IGV
CSB-MP319505IGV
CSB-EP319505IGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chandipura virus Glycoprotein G (G), partial CSB-YP319506CHN1
CSB-BP319506CHN1
CSB-MP319506CHN1
CSB-EP319506CHN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine protein kinase KIN1 (KIN1), partial CSB-YP319507SVG
CSB-EP319507SVG
CSB-BP319507SVG
CSB-MP319507SVG
CSB-EP319507SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Cytoplasmic envelopment protein 3 (UL99) CSB-YP319508HWV
CSB-BP319508HWV
CSB-MP319508HWV
CSB-EP319508HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atractaspis engaddensis Sarafotoxin-D CSB-YP319509AQO
CSB-EP319509AQO
CSB-BP319509AQO
CSB-MP319509AQO
CSB-EP319509AQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian cytomegalovirus Major DNA-binding protein (UL57), partial CSB-YP319510SGO
CSB-EP319510SGO
CSB-BP319510SGO
CSB-MP319510SGO
CSB-EP319510SGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus Malate synthase, glyoxysomal, partial CSB-YP319511BWD
CSB-EP319511BWD
CSB-BP319511BWD
CSB-MP319511BWD
CSB-EP319511BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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