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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Putative esterase ComA2 (yuxO) CSB-YP321213BRJ
CSB-EP321213BRJ
CSB-BP321213BRJ
CSB-MP321213BRJ
CSB-EP321213BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major fimbrial subunit (hifA) CSB-YP321214HSZ
CSB-EP321214HSZ
CSB-BP321214HSZ
CSB-MP321214HSZ
CSB-EP321214HSZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Limulus polyphemus Polyphemusin-2 CSB-YP321215LGC
CSB-EP321215LGC
CSB-BP321215LGC
CSB-MP321215LGC
CSB-EP321215LGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypocrea jecorina Phosphoglycerate kinase (pgk1) CSB-YP321216HYE
CSB-EP321216HYE
CSB-BP321216HYE
CSB-MP321216HYE
CSB-EP321216HYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum TGACG-sequence-specific DNA-binding protein TGA-1A (TGA1A) CSB-YP321217NHE
CSB-EP321217NHE
CSB-BP321217NHE
CSB-MP321217NHE
CSB-EP321217NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymphocytic choriomeningitis virus RNA-directed RNA polymerase L (L) CSB-YP321218LKZ
CSB-EP321218LKZ
CSB-BP321218LKZ
CSB-MP321218LKZ
CSB-EP321218LKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1-phosphatidylinositol phosphodiesterase CSB-BP321220BQJ
CSB-MP321220BQJ
CSB-EP321220BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 2 E1B protein, large T-antigen CSB-YP321221CCN
CSB-EP321221CCN
CSB-BP321221CCN
CSB-MP321221CCN
CSB-EP321221CCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Chlorophyll a-b binding protein 1C, chloroplastic (CAB1C), partial CSB-YP321222SZY1
CSB-EP321222SZY1
CSB-BP321222SZY1
CSB-MP321222SZY1
CSB-EP321222SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Pectinesterase 1 (PME1.9) CSB-YP321223SZY
CSB-EP321223SZY
CSB-BP321223SZY
CSB-MP321223SZY
CSB-EP321223SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Pertactin autotransporter (prn), partial CSB-YP321224BUA
CSB-BP321224BUA
CSB-MP321224BUA
CSB-EP321224BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Long-chain-fatty-acid--luciferin-component ligase (luxE) CSB-YP321225VFD
CSB-EP321225VFD
CSB-BP321225VFD
CSB-MP321225VFD
CSB-EP321225VFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA topoisomerase 3 (topB) CSB-YP321226ENV
CSB-EP321226ENV
CSB-BP321226ENV
CSB-MP321226ENV
CSB-EP321226ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Chalcone--flavonone isomerase (CHI) CSB-YP321227EUN
CSB-EP321227EUN
CSB-BP321227EUN
CSB-MP321227EUN
CSB-EP321227EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein fixA (fixA) CSB-YP321230RKN
CSB-EP321230RKN
CSB-BP321230RKN
CSB-MP321230RKN
CSB-EP321230RKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Muscle-specific protein 20 (Mp20) CSB-YP321231DLU
CSB-EP321231DLU
CSB-BP321231DLU
CSB-MP321231DLU
CSB-EP321231DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium sp. Uncharacterized 36 kDa protein CSB-YP321232HBL
CSB-EP321232HBL
CSB-BP321232HBL
CSB-MP321232HBL
CSB-EP321232HBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Vegetative-specific protein V4 (lmcB) CSB-YP321233DKK
CSB-EP321233DKK
CSB-BP321233DKK
CSB-MP321233DKK
CSB-EP321233DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 ICP47 protein (US12) CSB-YP321236HJX
CSB-EP321236HJX
CSB-BP321236HJX
CSB-MP321236HJX
CSB-EP321236HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Small capsomere-interacting protein (SCP) CSB-YP321237EFA
CSB-EP321237EFA
CSB-BP321237EFA
CSB-MP321237EFA
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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