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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Aspartate--tRNA ligase (aspS) CSB-YP322043ENV
CSB-EP322043ENV
CSB-BP322043ENV
CSB-MP322043ENV
CSB-EP322043ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Alanine--tRNA ligase, cytoplasmic, partial CSB-YP322044BTT
CSB-EP322044BTT
CSB-BP322044BTT
CSB-MP322044BTT
CSB-EP322044BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chironomus thummi thummi Histone H2B CSB-YP322045CIR
CSB-EP322045CIR
CSB-BP322045CIR
CSB-MP322045CIR
CSB-EP322045CIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Adenylosuccinate synthetase (purA) CSB-YP322046DKK
CSB-EP322046DKK
CSB-BP322046DKK
CSB-MP322046DKK
CSB-EP322046DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit a, sodium ion specific (atpB), partial CSB-YP322047PSI1
CSB-EP322047PSI1
CSB-BP322047PSI1
CSB-MP322047PSI1
CSB-EP322047PSI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis Glucose facilitated diffusion protein (glf), partial CSB-YP322048ZBM
CSB-EP322048ZBM
CSB-BP322048ZBM
CSB-MP322048ZBM
CSB-EP322048ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Lamin-L (II), partial CSB-YP322049XBE
CSB-EP322049XBE
CSB-BP322049XBE
CSB-MP322049XBE
CSB-EP322049XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Precorrin-6A reductase (cobK) CSB-YP322050PUH
CSB-EP322050PUH
CSB-BP322050PUH
CSB-MP322050PUH
CSB-EP322050PUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorghum bicolor Defensin-like protein 2 CSB-YP322051FJS
CSB-EP322051FJS
CSB-BP322051FJS
CSB-MP322051FJS
CSB-EP322051FJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichostrongylus colubriformis 11 kDa excretory-secretory protein CSB-YP322052TQF
CSB-EP322052TQF
CSB-BP322052TQF
CSB-MP322052TQF
CSB-EP322052TQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylose repressor (xylR) CSB-YP322053LAZ
CSB-EP322053LAZ
CSB-BP322053LAZ
CSB-MP322053LAZ
CSB-EP322053LAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Abutilon mosaic virus Replication enhancer protein (AC3, AL3) CSB-YP322054AAE
CSB-EP322054AAE
CSB-BP322054AAE
CSB-MP322054AAE
CSB-EP322054AAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Abutilon mosaic virus Replication-associated protein (AC1, AL1) CSB-YP322055AAE
CSB-EP322055AAE
CSB-BP322055AAE
CSB-MP322055AAE
CSB-EP322055AAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uptake hydrogenase large subunit (hoxG), partial CSB-YP322056DPX
CSB-EP322056DPX
CSB-BP322056DPX
CSB-MP322056DPX
CSB-EP322056DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Early transcription factor 70 kDa subunit (VETFS), partial CSB-YP322057FEY
CSB-EP322057FEY
CSB-BP322057FEY
CSB-MP322057FEY
CSB-EP322057FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Protein FPV063 (FPV063) CSB-YP322058FEY
CSB-EP322058FEY
CSB-BP322058FEY
CSB-MP322058FEY
CSB-EP322058FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Putative CC-type chemokine FPV061 (FPV061) CSB-YP322059FEY
CSB-EP322059FEY
CSB-BP322059FEY
CSB-MP322059FEY
CSB-EP322059FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus downei Cell surface antigen I/II (spaA), partial CSB-YP322060SMJ
CSB-BP322060SMJ
CSB-MP322060SMJ
CSB-EP322060SMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar filament outer layer protein (flaA) CSB-YP322061SJS
CSB-EP322061SJS
CSB-BP322061SJS
CSB-MP322061SJS
CSB-EP322061SJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar filament 33 kDa core protein CSB-YP322062SJS
CSB-EP322062SJS
CSB-BP322062SJS
CSB-MP322062SJS
CSB-EP322062SJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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