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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Western equine encephalitis virus Non-structural polyprotein, partial CSB-YP321171WAG
CSB-EP321171WAG
CSB-BP321171WAG
CSB-MP321171WAG
CSB-EP321171WAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human hepatitis A virus genotype IB Genome polyprotein, partial CSB-YP321172HJH
CSB-EP321172HJH
CSB-BP321172HJH
CSB-MP321172HJH
CSB-EP321172HJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Modification methylase BspRI (bspRIM) CSB-YP321173LRU
CSB-EP321173LRU
CSB-BP321173LRU
CSB-MP321173LRU
CSB-EP321173LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Arylamine N-acetyltransferase, pineal gland isozyme NAT-10 CSB-YP321174CH
CSB-EP321174CH
CSB-BP321174CH
CSB-MP321174CH
CSB-EP321174CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Convicilin (CVCA), partial CSB-YP321175EWE
CSB-EP321175EWE
CSB-BP321175EWE
CSB-MP321175EWE
CSB-EP321175EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Convicilin (CVCB) CSB-YP321176EWE
CSB-EP321176EWE
CSB-BP321176EWE
CSB-MP321176EWE
CSB-EP321176EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein CopA/IncA (copA) CSB-YP321177ENL
CSB-EP321177ENL
CSB-BP321177ENL
CSB-MP321177ENL
CSB-EP321177ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spisula solidissima G2/mitotic-specific cyclin-B CSB-YP321178SKE
CSB-EP321178SKE
CSB-BP321178SKE
CSB-MP321178SKE
CSB-EP321178SKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Replication regulatory protein RepA2 (repA2) CSB-YP321179ENV
CSB-EP321179ENV
CSB-BP321179ENV
CSB-MP321179ENV
CSB-EP321179ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Protein rep (rep) CSB-YP321180BQB
CSB-EP321180BQB
CSB-BP321180BQB
CSB-MP321180BQB
CSB-EP321180BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein sok (sok) CSB-YP321181ENL
CSB-EP321181ENL
CSB-BP321181ENL
CSB-MP321181ENL
CSB-EP321181ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antitoxin PemI (pemI) CSB-YP321182ENL
CSB-EP321182ENL
CSB-BP321182ENL
CSB-MP321182ENL
CSB-EP321182ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Carbamate kinase (arcC) CSB-YP321183EZX
CSB-EP321183EZX
CSB-BP321183EZX
CSB-MP321183EZX
CSB-EP321183EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposon Tn7 transposition protein tnsA (tnsA) CSB-YP321184ENL
CSB-EP321184ENL
CSB-BP321184ENL
CSB-MP321184ENL
CSB-EP321184ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cairina moschata Histone H2B CSB-YP321185CAG
CSB-EP321185CAG
CSB-BP321185CAG
CSB-MP321185CAG
CSB-EP321185CAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dermatophagoides pteronyssinus Mite allergen Der p 5 (DERP5) CSB-YP321186DJH
CSB-EP321186DJH
CSB-BP321186DJH
CSB-MP321186DJH
CSB-EP321186DJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isopentenyl transferase (tzs) CSB-YP321187AEW
CSB-EP321187AEW
CSB-BP321187AEW
CSB-MP321187AEW
CSB-EP321187AEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease P protein component 1 (rnp1) CSB-YP321188MEZ
CSB-EP321188MEZ
CSB-BP321188MEZ
CSB-MP321188MEZ
CSB-EP321188MEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii 50S ribosomal protein L30e (rpl30e) CSB-YP321189MNS
CSB-EP321189MNS
CSB-BP321189MNS
CSB-MP321189MNS
CSB-EP321189MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L15P (rpl15p) CSB-YP321191MEZ
CSB-EP321191MEZ
CSB-BP321191MEZ
CSB-MP321191MEZ
CSB-EP321191MEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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