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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cellodextrinase A (celA) CSB-YP321426ROT
CSB-EP321426ROT
CSB-BP321426ROT
CSB-MP321426ROT
CSB-EP321426ROT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Neuraminidase (NA), partial CSB-YP321427IJM
CSB-EP321427IJM
CSB-BP321427IJM
CSB-MP321427IJM
CSB-EP321427IJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Neuraminidase (NA), partial CSB-YP321428IJY
CSB-EP321428IJY
CSB-BP321428IJY
CSB-MP321428IJY
CSB-EP321428IJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endoglucanase 1 (Eg I) CSB-YP321429RHY
CSB-EP321429RHY
CSB-BP321429RHY
CSB-MP321429RHY
CSB-EP321429RHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter agglomerans Ice nucleation protein (iceE), partial CSB-EP321430EKP
CSB-BP321430EKP
CSB-MP321430EKP
CSB-EP321430EKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP321431FOB
CSB-EP321431FOB
CSB-BP321431FOB
CSB-MP321431FOB
CSB-EP321431FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemonchus contortus Cuticle collagen 3A3 (3A3) CSB-YP321432HSW
CSB-EP321432HSW
CSB-BP321432HSW
CSB-MP321432HSW
CSB-EP321432HSW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron-iron protein delta chain (anfG) CSB-YP321433DPX
CSB-EP321433DPX
CSB-BP321433DPX
CSB-MP321433DPX
CSB-EP321433DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Phosphoprotein (P) CSB-YP321434RAJ
CSB-EP321434RAJ
CSB-BP321434RAJ
CSB-MP321434RAJ
CSB-EP321434RAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Nodulin-21, partial CSB-YP321435GGV1
CSB-EP321435GGV1
CSB-BP321435GGV1
CSB-MP321435GGV1
CSB-EP321435GGV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Flagellar hook-associated protein 2 (fliD) CSB-YP321436SXB
CSB-EP321436SXB
CSB-BP321436SXB
CSB-MP321436SXB
CSB-EP321436SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Locupressin CSB-YP321437LHY
CSB-EP321437LHY
CSB-BP321437LHY
CSB-MP321437LHY
CSB-EP321437LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalaria pallida Lectin CSB-YP321438CSQ
CSB-EP321438CSQ
CSB-BP321438CSQ
CSB-MP321438CSQ
CSB-EP321438CSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Steroid receptor seven-up, isoform A (svp), partial CSB-YP321439DLU
CSB-EP321439DLU
CSB-BP321439DLU
CSB-MP321439DLU
CSB-EP321439DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Putative thiosulfate sulfurtransferase (cysA) CSB-YP321440SAL
CSB-EP321440SAL
CSB-BP321440SAL
CSB-MP321440SAL
CSB-EP321440SAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Flagellar basal body rod protein FlgB (flgB) CSB-YP321442SXB
CSB-EP321442SXB
CSB-BP321442SXB
CSB-MP321442SXB
CSB-EP321442SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apical membrane antigen 1 (AMA-1), partial CSB-YP321443EWO
CSB-EP321443EWO
CSB-BP321443EWO
CSB-MP321443EWO
CSB-EP321443EWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin-activating lysine-acyltransferase lktC (lktC) CSB-YP321444AYK
CSB-EP321444AYK
CSB-BP321444AYK
CSB-MP321444AYK
CSB-EP321444AYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pesticidal crystal protein cry4Aa (cry4Aa), partial CSB-YP321445BCZ
CSB-EP321445BCZ
CSB-BP321445BCZ
CSB-MP321445BCZ
CSB-EP321445BCZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus RNA-directed RNA polymerase 2a (ORF2a), partial CSB-YP321446CTX
CSB-EP321446CTX
CSB-BP321446CTX
CSB-MP321446CTX
CSB-EP321446CTX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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