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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis RNA polymerase sigma-G factor (sigG) CSB-YP324868BRJ
CSB-EP324868BRJ
CSB-BP324868BRJ
CSB-MP324868BRJ
CSB-EP324868BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea 30S ribosomal protein 1, chloroplastic (RPS30) CSB-YP324869FKI
CSB-EP324869FKI
CSB-BP324869FKI
CSB-MP324869FKI
CSB-EP324869FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aipysurus laevis Short neurotoxin C CSB-YP324870AFL
CSB-EP324870AFL
CSB-BP324870AFL
CSB-MP324870AFL
CSB-EP324870AFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Torpedo californica SITS-binding protein, partial CSB-YP324871TOT1
CSB-EP324871TOT1
CSB-BP324871TOT1
CSB-MP324871TOT1
CSB-EP324871TOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Ferritin-1, chloroplastic CSB-YP324872EWE
CSB-EP324872EWE
CSB-BP324872EWE
CSB-MP324872EWE
CSB-EP324872EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta Juvenile hormone esterase CSB-YP324873MPV
CSB-EP324873MPV
CSB-BP324873MPV
CSB-MP324873MPV
CSB-EP324873MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acheta domesticus Subesophageal ganglion pentapeptide CSB-YP324874ABD
CSB-EP324874ABD
CSB-BP324874ABD
CSB-MP324874ABD
CSB-EP324874ABD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Mannose-binding protein A (Mbl1) CSB-YP324875RA
CSB-EP324875RA
CSB-BP324875RA
CSB-MP324875RA
CSB-EP324875RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovophis okinavensis Thrombin-like enzyme okinaxobin-1 CSB-YP324876OCY
CSB-EP324876OCY
CSB-BP324876OCY
CSB-MP324876OCY
CSB-EP324876OCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trametes versicolor Ligninase B, partial CSB-YP324877TPK
CSB-EP324877TPK
CSB-BP324877TPK
CSB-MP324877TPK
CSB-EP324877TPK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thunnus thynnus NAD-specific glutamate dehydrogenase CSB-YP324878TEK
CSB-EP324878TEK
CSB-BP324878TEK
CSB-MP324878TEK
CSB-EP324878TEK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Myb-related protein Zm1 CSB-YP324879ZAX
CSB-EP324879ZAX
CSB-BP324879ZAX
CSB-MP324879ZAX
CSB-EP324879ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Myb-related protein Hv1 (MYB1) CSB-YP324880HWQ
CSB-EP324880HWQ
CSB-BP324880HWQ
CSB-MP324880HWQ
CSB-EP324880HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L5 (rplE) CSB-YP324881EZA
CSB-EP324881EZA
CSB-BP324881EZA
CSB-MP324881EZA
CSB-EP324881EZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse H-2 class II histocompatibility antigen, E-Q beta chain, partial CSB-YP324882MO
CSB-EP324882MO
CSB-BP324882MO
CSB-MP324882MO
CSB-EP324882MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein repB (repB) CSB-YP324883LMR
CSB-EP324883LMR
CSB-BP324883LMR
CSB-MP324883LMR
CSB-EP324883LMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae PHO85 cyclin PHO80 (PHO80) CSB-YP324884SVG
CSB-EP324884SVG
CSB-BP324884SVG
CSB-MP324884SVG
CSB-EP324884SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae FK506-binding protein 1 (FPR1) CSB-YP324885SVG
CSB-EP324885SVG
CSB-BP324885SVG
CSB-MP324885SVG
CSB-EP324885SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans Mobilization protein mobL (mobL) CSB-YP324886TOA
CSB-EP324886TOA
CSB-BP324886TOA
CSB-MP324886TOA
CSB-EP324886TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II 12 kDa extrinsic protein (psbU) CSB-YP324888PIB
CSB-EP324888PIB
CSB-BP324888PIB
CSB-MP324888PIB
CSB-EP324888PIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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