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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Glycerol uptake/efflux facilitator protein (FPS1), partial CSB-YP326102SVG1
CSB-EP326102SVG1
CSB-BP326102SVG1
CSB-MP326102SVG1
CSB-EP326102SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA mismatch repair protein MutS (mutS), partial CSB-YP326103ENV
CSB-EP326103ENV
CSB-BP326103ENV
CSB-MP326103ENV
CSB-EP326103ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Methionine--tRNA ligase (metG), partial CSB-YP326104GFM
CSB-EP326104GFM
CSB-BP326104GFM
CSB-MP326104GFM
CSB-EP326104GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Transcriptional activator protein anr (anr) CSB-YP326105EZX
CSB-EP326105EZX
CSB-BP326105EZX
CSB-MP326105EZX
CSB-EP326105EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Type-2 restriction enzyme BamHI (bamHIR) CSB-YP326106BQB
CSB-EP326106BQB
CSB-BP326106BQB
CSB-MP326106BQB
CSB-EP326106BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Low calcium response locus protein H (lcrH) CSB-YP326108YAH
CSB-EP326108YAH
CSB-BP326108YAH
CSB-MP326108YAH
CSB-EP326108YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Transmembrane regulatory protein toxS (toxS), partial CSB-YP326109VEX
CSB-EP326109VEX
CSB-BP326109VEX
CSB-MP326109VEX
CSB-EP326109VEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 3-isopropylmalate dehydrogenase (leuB) CSB-YP326110LOS
CSB-EP326110LOS
CSB-BP326110LOS
CSB-MP326110LOS
CSB-EP326110LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus oryzae Nuclease S1 (nucS) CSB-YP326111DPA
CSB-EP326111DPA
CSB-BP326111DPA
CSB-MP326111DPA
CSB-EP326111DPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lycodes polaris Ice-structuring protein LP CSB-YP326112LKP
CSB-EP326112LKP
CSB-BP326112LKP
CSB-MP326112LKP
CSB-EP326112LKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sperm-specific basic nuclear protein SP4 (sp4-a) CSB-YP326113XBE
CSB-EP326113XBE
CSB-BP326113XBE
CSB-MP326113XBE
CSB-EP326113XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum 30S ribosomal protein S12, chloroplastic (rps12-A) CSB-YP326114TQN
CSB-EP326114TQN
CSB-BP326114TQN
CSB-MP326114TQN
CSB-EP326114TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Flagellar motor switch phosphatase FliY (fliY) CSB-YP326115BRJ
CSB-EP326115BRJ
CSB-BP326115BRJ
CSB-MP326115BRJ
CSB-EP326115BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus Alkaline nuclease (ALK-EXO) CSB-YP326116OEU
CSB-EP326116OEU
CSB-BP326116OEU
CSB-MP326116OEU
CSB-EP326116OEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cyclin-dependent kinase A-1 (CDKA-1) CSB-YP326117DOA
CSB-EP326117DOA
CSB-BP326117DOA
CSB-MP326117DOA
CSB-EP326117DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpx (vpx) CSB-YP326118HLV
CSB-EP326118HLV
CSB-BP326118HLV
CSB-MP326118HLV
CSB-EP326118HLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Fusaric acid resistance protein fusB (fusB) CSB-YP326119BXR
CSB-EP326119BXR
CSB-BP326119BXR
CSB-MP326119BXR
CSB-EP326119BXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein J (nodJ), partial CSB-YP326120RCT1
CSB-EP326120RCT1
CSB-BP326120RCT1
CSB-MP326120RCT1
CSB-EP326120RCT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Hemagglutinin/proteinase (hap) CSB-YP326121VEX
CSB-EP326121VEX
CSB-BP326121VEX
CSB-MP326121VEX
CSB-EP326121VEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acriflavine resistance protein F (acrF), partial CSB-YP326122ENV
CSB-EP326122ENV
CSB-BP326122ENV
CSB-MP326122ENV
CSB-EP326122ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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