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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vaccinia virus Uncharacterized 12.9 kDa protein (E ORF F) CSB-YP324952VAA
CSB-EP324952VAA
CSB-BP324952VAA
CSB-MP324952VAA
CSB-EP324952VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.8 kDa protein (F ORF F) CSB-YP324953VAA
CSB-EP324953VAA
CSB-BP324953VAA
CSB-MP324953VAA
CSB-EP324953VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.4 kDa protein (K ORF A) CSB-YP324954VAA
CSB-EP324954VAA
CSB-BP324954VAA
CSB-MP324954VAA
CSB-EP324954VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Anthranilate synthase component II (trpG) CSB-YP324955EZX
CSB-EP324955EZX
CSB-BP324955EZX
CSB-MP324955EZX
CSB-EP324955EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Anthranilate synthase component 1 (trpE) CSB-YP324956EZX
CSB-EP324956EZX
CSB-BP324956EZX
CSB-MP324956EZX
CSB-EP324956EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Small COPII coat GTPase SAR1 (SAR1) CSB-YP324957SVG
CSB-EP324957SVG
CSB-BP324957SVG
CSB-MP324957SVG
CSB-EP324957SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Nitrogenase molybdenum-iron protein beta chain (nifK), partial CSB-YP324958BVW
CSB-EP324958BVW
CSB-BP324958BVW
CSB-MP324958BVW
CSB-EP324958BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium pseudotuberculosis Phospholipase D (pld) CSB-YP324959CQM
CSB-EP324959CQM
CSB-BP324959CQM
CSB-MP324959CQM
CSB-EP324959CQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuticle collagen 13 (col-13) CSB-YP324960CXY
CSB-EP324960CXY
CSB-BP324960CXY
CSB-MP324960CXY
CSB-EP324960CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Early transcription factor 82 kDa subunit (VETFL), partial CSB-YP324961VAA
CSB-EP324961VAA
CSB-BP324961VAA
CSB-MP324961VAA
CSB-EP324961VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Serine/threonine-protein phosphatase PP1 (bimG) CSB-YP324962EKF
CSB-EP324962EKF
CSB-BP324962EKF
CSB-MP324962EKF
CSB-EP324962EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida HTH-type transcriptional regulator CatR (catR) CSB-YP324963FFZ
CSB-EP324963FFZ
CSB-BP324963FFZ
CSB-MP324963FFZ
CSB-EP324963FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-oxoglutarate dehydrogenase E1 component (sucA), partial CSB-YP324964DPX
CSB-EP324964DPX
CSB-BP324964DPX
CSB-MP324964DPX
CSB-EP324964DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Arylsulfatase (atsA) CSB-YP324965EKN
CSB-EP324965EKN
CSB-BP324965EKN
CSB-MP324965EKN
CSB-EP324965EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Enterotoxin type D (entD) CSB-YP324966FKZ
CSB-BP324966FKZ
CSB-MP324966FKZ
CSB-EP324966FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paralithodes camtschaticus Collagenolytic protease 36 kDa A CSB-YP324967PCF
CSB-EP324967PCF
CSB-BP324967PCF
CSB-MP324967PCF
CSB-EP324967PCF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowl adenovirus A serotype 1 Uncharacterized protein ORF17 (17) CSB-YP324968FCL
CSB-EP324968FCL
CSB-BP324968FCL
CSB-MP324968FCL
CSB-EP324968FCL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowl adenovirus A serotype 1 Putative Ig-like domain-containing protein ORF10 (10) CSB-YP324969FCL
CSB-EP324969FCL
CSB-BP324969FCL
CSB-MP324969FCL
CSB-EP324969FCL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit RLA class II histocompatibility antigen, DP alpha-1 chain, partial CSB-YP324970RB
CSB-EP324970RB
CSB-BP324970RB
CSB-MP324970RB
CSB-EP324970RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ig gamma-2A chain C region (Igg-2a) CSB-YP324971RA
CSB-EP324971RA
CSB-BP324971RA
CSB-MP324971RA
CSB-EP324971RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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