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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Vaccinia virus 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (A44L) CSB-YP325013VAA
CSB-EP325013VAA
CSB-BP325013VAA
CSB-MP325013VAA
CSB-EP325013VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C19/B25 (B25R) CSB-YP325014VAA
CSB-EP325014VAA
CSB-BP325014VAA
CSB-MP325014VAA
CSB-EP325014VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit C-X-C chemokine receptor type 1 (CXCR1), partial CSB-YP325015RB1
CSB-EP325015RB1
CSB-BP325015RB1
CSB-MP325015RB1
CSB-EP325015RB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Methyl-coenzyme M reductase II subunit gamma (mrtG) CSB-YP325016MSR
CSB-EP325016MSR
CSB-BP325016MSR
CSB-MP325016MSR
CSB-EP325016MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.2 kDa protein (C ORF C) CSB-YP325017VAA
CSB-EP325017VAA
CSB-BP325017VAA
CSB-MP325017VAA
CSB-EP325017VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caprine arthritis encephalitis virus Probable Vpr-like protein (tat) CSB-YP325018CDG
CSB-EP325018CDG
CSB-BP325018CDG
CSB-MP325018CDG
CSB-EP325018CDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phytoene dehydrogenase (crtI), partial CSB-YP325019SSO
CSB-EP325019SSO
CSB-BP325019SSO
CSB-MP325019SSO
CSB-EP325019SSO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Acyl-CoA desaturase 1 (OLE1), partial CSB-YP325020SVG1
CSB-EP325020SVG1
CSB-BP325020SVG1
CSB-MP325020SVG1
CSB-EP325020SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin CSB-YP325021TPY
CSB-EP325021TPY
CSB-BP325021TPY
CSB-MP325021TPY
CSB-EP325021TPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP325022NFZ
CSB-EP325022NFZ
CSB-BP325022NFZ
CSB-MP325022NFZ
CSB-EP325022NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cobyric acid synthase (cobQ) CSB-YP325023MSD
CSB-EP325023MSD
CSB-BP325023MSD
CSB-MP325023MSD
CSB-EP325023MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant C-factor (csgA) CSB-YP325024MXA
CSB-EP325024MXA
CSB-BP325024MXA
CSB-MP325024MXA
CSB-EP325024MXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micromonospora echinospora Calichemicin antitumor antibiotic biosynthesis protein CSB-YP325025MGB
CSB-EP325025MGB
CSB-BP325025MGB
CSB-MP325025MGB
CSB-EP325025MGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Probable endopeptidase p60 (iap) CSB-YP325026LPY
CSB-EP325026LPY
CSB-BP325026LPY
CSB-MP325026LPY
CSB-EP325026LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrococcus luteus Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP325027MTO
CSB-EP325027MTO
CSB-BP325027MTO
CSB-MP325027MTO
CSB-EP325027MTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrococcus luteus DNA polymerase III subunit beta (dnaN) CSB-YP325028MTO
CSB-EP325028MTO
CSB-BP325028MTO
CSB-MP325028MTO
CSB-EP325028MTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella sonnei Colicin-E1 (cea), partial CSB-YP325029SGH1
CSB-EP325029SGH1
CSB-BP325029SGH1
CSB-MP325029SGH1
CSB-EP325029SGH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysis protein for colicin E1* (kil) CSB-YP325030SGH
CSB-EP325030SGH
CSB-BP325030SGH
CSB-MP325030SGH
CSB-EP325030SGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Polyadenylate-binding protein (pAbp), partial CSB-YP325031DLU
CSB-EP325031DLU
CSB-BP325031DLU
CSB-MP325031DLU
CSB-EP325031DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Metallothionein expression activator (ACE2), partial CSB-YP325032SVG
CSB-EP325032SVG
CSB-BP325032SVG
CSB-MP325032SVG
CSB-EP325032SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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