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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodopseudomonas acidophila Light-harvesting protein B-800/850 beta chain, partial CSB-YP326662RDT
CSB-EP326662RDT
CSB-BP326662RDT
CSB-MP326662RDT
CSB-EP326662RDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homeobox protein ceh-19 (ceh-19) CSB-YP326663CXY
CSB-EP326663CXY
CSB-BP326663CXY
CSB-MP326663CXY
CSB-EP326663CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus DNA polymerase 1 (dpo1), partial CSB-YP326664FPM
CSB-EP326664FPM
CSB-BP326664FPM
CSB-MP326664FPM
CSB-EP326664FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vigna unguiculata 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP326665VFM
CSB-EP326665VFM
CSB-BP326665VFM
CSB-MP326665VFM
CSB-EP326665VFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator CPE0189 (CPE0189) CSB-YP326666CMB
CSB-EP326666CMB
CSB-BP326666CMB
CSB-MP326666CMB
CSB-EP326666CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Ribosomal protein S1, mitochondrial (RPS1) CSB-YP326667MCB
CSB-EP326667MCB
CSB-BP326667MCB
CSB-MP326667MCB
CSB-EP326667MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Lactonizing lipase (lip) CSB-YP326668EZX
CSB-EP326668EZX
CSB-BP326668EZX
CSB-MP326668EZX
CSB-EP326668EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aspartate ammonia-lyase (ansB) CSB-YP326669BRJ
CSB-EP326669BRJ
CSB-BP326669BRJ
CSB-MP326669BRJ
CSB-EP326669BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Dipeptide transport ATP-binding protein DppD (dppD) CSB-YP326670BRJ
CSB-EP326670BRJ
CSB-BP326670BRJ
CSB-MP326670BRJ
CSB-EP326670BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin soy (soyB) CSB-YP326671SUW
CSB-EP326671SUW
CSB-BP326671SUW
CSB-MP326671SUW
CSB-EP326671SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stage IV sporulation protein FA (spoIVFA), partial CSB-YP326672BRJ1
CSB-EP326672BRJ1
CSB-BP326672BRJ1
CSB-MP326672BRJ1
CSB-EP326672BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Indole-3-glycerol phosphate synthase (trpC) CSB-YP326673MSQ
CSB-EP326673MSQ
CSB-BP326673MSQ
CSB-MP326673MSQ
CSB-EP326673MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Guanine nucleotide-binding protein alpha-1 subunit (GPA1) CSB-YP326674SZY
CSB-EP326674SZY
CSB-BP326674SZY
CSB-MP326674SZY
CSB-EP326674SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Aliphatic amidase expression-regulating protein (amiC) CSB-YP326675EZX
CSB-EP326675EZX
CSB-BP326675EZX
CSB-MP326675EZX
CSB-EP326675EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human respiratory syncytial virus A Major surface glycoprotein G (G), partial CSB-YP326676HPT1
CSB-EP326676HPT1
CSB-BP326676HPT1
CSB-MP326676HPT1
CSB-EP326676HPT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Astasia longa DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP326677APY
CSB-EP326677APY
CSB-BP326677APY
CSB-MP326677APY
CSB-EP326677APY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vigna unguiculata subsp. unguiculata Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP326678VCX
CSB-EP326678VCX
CSB-BP326678VCX
CSB-MP326678VCX
CSB-EP326678VCX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P21 Excisionase (xis) CSB-YP326679EDF
CSB-EP326679EDF
CSB-BP326679EDF
CSB-MP326679EDF
CSB-EP326679EDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Chlorocatechol 1,2-dioxygenase (tcbC) CSB-YP326680PVT
CSB-EP326680PVT
CSB-BP326680PVT
CSB-MP326680PVT
CSB-EP326680PVT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. UPF0065 protein in tcbD-tcbE intergenic region CSB-YP326681PVT
CSB-EP326681PVT
CSB-BP326681PVT
CSB-MP326681PVT
CSB-EP326681PVT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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