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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Reaction center protein L chain (pufL), partial CSB-YP361701RLX1
CSB-EP361701RLX1
CSB-BP361701RLX1
CSB-MP361701RLX1
CSB-EP361701RLX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Replication protein 1a (ORF1a), partial CSB-YP361702CUC
CSB-EP361702CUC
CSB-BP361702CUC
CSB-MP361702CUC
CSB-EP361702CUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variant surface glycoprotein ILTAT 1.2 CSB-YP361703TQV
CSB-EP361703TQV
CSB-BP361703TQV
CSB-MP361703TQV
CSB-EP361703TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variant surface glycoprotein AnTaT 1.1 CSB-YP361704TQV
CSB-EP361704TQV
CSB-BP361704TQV
CSB-MP361704TQV
CSB-EP361704TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variant surface glycoprotein ANTAT 1.8 CSB-YP361705TQV
CSB-EP361705TQV
CSB-BP361705TQV
CSB-MP361705TQV
CSB-EP361705TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage Mu Repressor protein CI (CI) CSB-YP361706EDA
CSB-EP361706EDA
CSB-BP361706EDA
CSB-MP361706EDA
CSB-EP361706EDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage Mu Host-nuclease inhibitor protein gam (gam) CSB-YP361707EDA
CSB-EP361707EDA
CSB-BP361707EDA
CSB-MP361707EDA
CSB-EP361707EDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suid herpesvirus 1 Glycoprotein GIII, partial CSB-YP361708SRH
CSB-EP361708SRH
CSB-BP361708SRH
CSB-MP361708SRH
CSB-EP361708SRH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus chinensis Rhizopuspepsin CSB-YP361709RDB
CSB-EP361709RDB
CSB-BP361709RDB
CSB-MP361709RDB
CSB-EP361709RDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae General negative regulator of transcription subunit 2 (CDC36) CSB-YP361710SVG
CSB-EP361710SVG
CSB-BP361710SVG
CSB-MP361710SVG
CSB-EP361710SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae General negative regulator of transcription subunit 3 (NOT3), partial CSB-YP361711SVG
CSB-EP361711SVG
CSB-BP361711SVG
CSB-MP361711SVG
CSB-EP361711SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin-conjugating enzyme E2 2 (RAD6) CSB-YP361712SVG
CSB-EP361712SVG
CSB-BP361712SVG
CSB-MP361712SVG
CSB-EP361712SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein MET17 (MET17) CSB-YP361713SVG
CSB-EP361713SVG
CSB-BP361713SVG
CSB-MP361713SVG
CSB-EP361713SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein p49 (p49) CSB-YP361714FOI
CSB-EP361714FOI
CSB-BP361714FOI
CSB-MP361714FOI
CSB-EP361714FOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Chemotaxis protein CheW (cheW) CSB-YP361715SXB
CSB-EP361715SXB
CSB-BP361715SXB
CSB-MP361715SXB
CSB-EP361715SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Allophycocyanin alpha chain (apcA) CSB-YP361716FPZ
CSB-EP361716FPZ
CSB-BP361716FPZ
CSB-MP361716FPZ
CSB-EP361716FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core (apcC) CSB-YP361717FPZ
CSB-EP361717FPZ
CSB-BP361717FPZ
CSB-MP361717FPZ
CSB-EP361717FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal protein MC1 CSB-YP361718MSL
CSB-EP361718MSL
CSB-BP361718MSL
CSB-MP361718MSL
CSB-EP361718MSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Nitrogenase iron protein (nifH) CSB-YP361719BVW
CSB-EP361719BVW
CSB-BP361719BVW
CSB-MP361719BVW
CSB-EP361719BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein (nifH) CSB-YP361720MSD
CSB-EP361720MSD
CSB-BP361720MSD
CSB-MP361720MSD
CSB-EP361720MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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