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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Triticum aestivum Serine carboxypeptidase 2 (CBP2) CSB-YP362523TQN
CSB-EP362523TQN
CSB-BP362523TQN
CSB-MP362523TQN
CSB-EP362523TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA-binding protein HU 1 (hupA) CSB-YP362524BRJ
CSB-EP362524BRJ
CSB-BP362524BRJ
CSB-MP362524BRJ
CSB-EP362524BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ricinus communis RuBisCO large subunit-binding protein subunit alpha CSB-YP362525RMM
CSB-EP362525RMM
CSB-BP362525RMM
CSB-MP362525RMM
CSB-EP362525RMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Chorion class A protein L11 CSB-YP362526BTT
CSB-EP362526BTT
CSB-BP362526BTT
CSB-MP362526BTT
CSB-EP362526BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Chorion class B protein L12 CSB-YP362527BTT
CSB-EP362527BTT
CSB-BP362527BTT
CSB-MP362527BTT
CSB-EP362527BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Chorion class CB protein M5H4 CSB-YP362528BTT
CSB-EP362528BTT
CSB-BP362528BTT
CSB-MP362528BTT
CSB-EP362528BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoenolpyruvate-protein phosphotransferase (ptsI) CSB-YP362529ENV
CSB-EP362529ENV
CSB-BP362529ENV
CSB-MP362529ENV
CSB-EP362529ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division inhibitor SulA (sulA) CSB-YP362530SYN
CSB-EP362530SYN
CSB-BP362530SYN
CSB-MP362530SYN
CSB-EP362530SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Cell division inhibitor SulA (sulA) CSB-YP362531EKN
CSB-EP362531EKN
CSB-BP362531EKN
CSB-MP362531EKN
CSB-EP362531EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Accipiter gentilis Hemoglobin subunit alpha-A (HBAA) CSB-YP362532AAS
CSB-EP362532AAS
CSB-BP362532AAS
CSB-MP362532AAS
CSB-EP362532AAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum-pterin-binding protein 2 (mopII) CSB-YP362533CMA
CSB-EP362533CMA
CSB-BP362533CMA
CSB-MP362533CMA
CSB-EP362533CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Excisase A (xisA) CSB-YP362534NHR
CSB-EP362534NHR
CSB-BP362534NHR
CSB-MP362534NHR
CSB-EP362534NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Nodulin-27 CSB-YP362535GGV
CSB-EP362535GGV
CSB-BP362535GGV
CSB-MP362535GGV
CSB-EP362535GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YuaZ (yuaZ) CSB-YP362536ENV
CSB-EP362536ENV
CSB-BP362536ENV
CSB-MP362536ENV
CSB-EP362536ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uca pugilator Pigment-dispersing hormone CSB-YP362537UAA
CSB-EP362537UAA
CSB-BP362537UAA
CSB-MP362537UAA
CSB-EP362537UAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphocarrier protein HPr (ptsH) CSB-YP362538BRJ
CSB-BP362538BRJ
CSB-MP362538BRJ
CSB-EP362538BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Granzyme F (Gzmf) CSB-YP362539MO
CSB-EP362539MO
CSB-BP362539MO
CSB-MP362539MO
CSB-EP362539MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Granzyme E (Gzme) CSB-YP362540MO
CSB-EP362540MO
CSB-BP362540MO
CSB-MP362540MO
CSB-EP362540MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein 10 (nodX), partial CSB-YP362541RKR
CSB-EP362541RKR
CSB-BP362541RKR
CSB-MP362541RKR
CSB-EP362541RKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglycerate kinase A, partial CSB-YP362542TQV
CSB-EP362542TQV
CSB-BP362542TQV
CSB-MP362542TQV
CSB-EP362542TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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