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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli L (+)-tartrate dehydratase subunit alpha CSB-YP361641ENV
CSB-EP361641ENV
CSB-BP361641ENV
CSB-MP361641ENV
CSB-EP361641ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrate utilization protein B (citB) CSB-YP361642ENL
CSB-EP361642ENL
CSB-BP361642ENL
CSB-MP361642ENL
CSB-EP361642ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef (nef) CSB-YP361643HKL
CSB-EP361643HKL
CSB-BP361643HKL
CSB-MP361643HKL
CSB-EP361643HKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef (nef) CSB-YP361644HLC
CSB-EP361644HLC
CSB-BP361644HLC
CSB-MP361644HLC
CSB-EP361644HLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype A Protein Nef (nef) CSB-YP361645HKH
CSB-EP361645HKH
CSB-BP361645HKH
CSB-MP361645HKH
CSB-EP361645HKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Nef (nef) CSB-YP361646SGZ
CSB-EP361646SGZ
CSB-BP361646SGZ
CSB-MP361646SGZ
CSB-EP361646SGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus agm.vervet Protein Nef (nef) CSB-YP361647SHH
CSB-EP361647SHH
CSB-BP361647SHH
CSB-MP361647SHH
CSB-EP361647SHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP361648HKL
CSB-EP361648HKL
CSB-BP361648HKL
CSB-MP361648HKL
CSB-EP361648HKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype U Protein Rev (rev) CSB-YP361649HLR
CSB-EP361649HLR
CSB-BP361649HLR
CSB-MP361649HLR
CSB-EP361649HLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Rev (rev) CSB-YP361650HLM
CSB-EP361650HLM
CSB-BP361650HLM
CSB-MP361650HLM
CSB-EP361650HLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP361651HKW
CSB-EP361651HKW
CSB-BP361651HKW
CSB-MP361651HKW
CSB-EP361651HKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Rev (rev) CSB-YP361652HLY
CSB-EP361652HLY
CSB-BP361652HLY
CSB-MP361652HLY
CSB-EP361652HLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus agm.vervet Protein Rev (rev) CSB-YP361653SHH
CSB-EP361653SHH
CSB-BP361653SHH
CSB-MP361653SHH
CSB-EP361653SHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Envelope glycoprotein gp160 (env), partial CSB-YP361654HLC
CSB-EP361654HLC
CSB-BP361654HLC
CSB-MP361654HLC
CSB-EP361654HLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Envelope glycoprotein gp160 (env), partial CSB-YP361655HKO
CSB-EP361655HKO
CSB-BP361655HKO
CSB-MP361655HKO
CSB-EP361655HKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype A Envelope glycoprotein gp160 (env), partial CSB-YP361656HKH
CSB-EP361656HKH
CSB-BP361656HKH
CSB-MP361656HKH
CSB-EP361656HKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Envelope glycoprotein gp160 (env), partial CSB-YP361657HLY
CSB-EP361657HLY
CSB-BP361657HLY
CSB-MP361657HLY
CSB-EP361657HLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus agm.vervet Envelope glycoprotein gp160 (env), partial CSB-YP361658SHH
CSB-EP361658SHH
CSB-BP361658SHH
CSB-MP361658SHH
CSB-EP361658SHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag polyprotein (gag) CSB-YP361659HKO
CSB-EP361659HKO
CSB-BP361659HKO
CSB-MP361659HKO
CSB-EP361659HKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag polyprotein (gag) CSB-YP361660HLG
CSB-EP361660HLG
CSB-BP361660HLG
CSB-MP361660HLG
CSB-EP361660HLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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