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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida Trans-O-hydroxybenzylidenepyruvate hydratase-aldolase (nahE) CSB-YP363035FFZ
CSB-EP363035FFZ
CSB-BP363035FFZ
CSB-MP363035FFZ
CSB-EP363035FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosome hibernation promoting factor (hpf) CSB-YP363036FGB
CSB-EP363036FGB
CSB-BP363036FGB
CSB-MP363036FGB
CSB-EP363036FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Uncharacterized protein PP_0002 (PP_0002) CSB-YP363037FGB
CSB-EP363037FGB
CSB-BP363037FGB
CSB-MP363037FGB
CSB-EP363037FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable chromosome-partitioning protein parB (parB) CSB-YP363038FGB
CSB-EP363038FGB
CSB-BP363038FGB
CSB-MP363038FGB
CSB-EP363038FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida LexA repressor 1 (lexA1) CSB-YP363039FGB
CSB-EP363039FGB
CSB-BP363039FGB
CSB-MP363039FGB
CSB-EP363039FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Alkyl hydroperoxide reductase subunit F (ahpF), partial CSB-YP363040FGB
CSB-EP363040FGB
CSB-BP363040FGB
CSB-MP363040FGB
CSB-EP363040FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L7/L12 (rplL) CSB-YP363041FGB
CSB-EP363041FGB
CSB-BP363041FGB
CSB-MP363041FGB
CSB-EP363041FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 50S ribosomal protein L20 (rplT) CSB-YP363042FGP
CSB-EP363042FGP
CSB-BP363042FGP
CSB-MP363042FGP
CSB-EP363042FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L34 (rpmH) CSB-YP363043FGB
CSB-EP363043FGB
CSB-BP363043FGB
CSB-MP363043FGB
CSB-EP363043FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 50S ribosomal protein L35 (rpmI) CSB-YP363044FGP
CSB-EP363044FGP
CSB-BP363044FGP
CSB-MP363044FGP
CSB-EP363044FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S21 (rpsU) CSB-YP363045FFZ
CSB-EP363045FFZ
CSB-BP363045FFZ
CSB-MP363045FFZ
CSB-EP363045FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribonuclease P protein component (rnpA) CSB-YP363046FFZ
CSB-EP363046FFZ
CSB-BP363046FFZ
CSB-MP363046FFZ
CSB-EP363046FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. 1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase (doxE) CSB-YP363047PVJ
CSB-EP363047PVJ
CSB-BP363047PVJ
CSB-MP363047PVJ
CSB-EP363047PVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida RNA polymerase sigma-54 factor (rpoN) CSB-YP363048FGB
CSB-EP363048FGB
CSB-BP363048FGB
CSB-MP363048FGB
CSB-EP363048FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein TolB (tolB), partial CSB-YP363049FFZ
CSB-EP363049FFZ
CSB-BP363049FFZ
CSB-MP363049FFZ
CSB-EP363049FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA modification GTPase MnmE (mnmE) CSB-YP363050FFZ
CSB-EP363050FFZ
CSB-BP363050FFZ
CSB-MP363050FFZ
CSB-EP363050FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. UPF0065 protein in clcB-clcD intergenic region CSB-YP363051FGG
CSB-EP363051FGG
CSB-BP363051FGG
CSB-MP363051FGG
CSB-EP363051FGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Putative membrane protein insertion efficiency factor CSB-YP363052FFZ
CSB-EP363052FFZ
CSB-BP363052FFZ
CSB-MP363052FFZ
CSB-EP363052FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Quinohemoprotein amine dehydrogenase subunit gamma (qhnDH) CSB-YP363053FFZ
CSB-EP363053FFZ
CSB-BP363053FFZ
CSB-MP363053FFZ
CSB-EP363053FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mercuric resistance operon regulatory protein (merR) CSB-YP363054FFT
CSB-EP363054FFT
CSB-BP363054FFT
CSB-MP363054FFT
CSB-EP363054FFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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